New insights on gonadal growth factors of Lambari, Astyanax altiparanae: basic knowledge and development of biotechnological strategies to delay puberty

• Grant number: 22/10389-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2022
• Status: Discontinued
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering
• Principal Investigator: Rafael Henrique Nóbrega
• Grantee: Amanda de Oliveira Ribeiro
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 21/06742-5 - New insights on gonadal signals (growth factors and lncRNAs) that regulate gonadal maturation in fish: basic knowledge and development of biotechnological strategies to delay puberty, AP.JP2
• Associated scholarship(s): 24/15270-8 - How do fish spermatogonial cells decide to shift from self-renewal to proliferation/differentiation on the onset of puberty?, BE.EP.PD

Abstract

The overarching goal of this research is to investigate new paracrine factors and indentify long non-coding RNAs (lncRNAs) that regulate SSC activity, and develop biotechnological strategies to delay puberty in fish. Early sexual maturation of males is still one the major problems limiting the further development of Aquaculture in the world. Sexual precocity is an undesirable trait in fish which is correlated with negative aspects such as reduced growth and lower feed conversion. In males, the onset of puberty is generally associated with a switch in SSC activity, from self-renewal to differentiation towards meiosis. Therefore, understanding the mechanisms controlling the switch in SSC activity is the basis for developing approaches to control precocious puberty in males. Focusing on new growth factors and lncRNAs, functional studies that combine in vivo approaches and gain-of-function ex vivo models will be employed. Functional studies will be evaluated with a combination of histomorphometric, biochemical, and molecular approaches. In this project, Lambari (Astyanax altiparanae), a Neotropical model and promising species for Brazilian Aquaculture, and Zebrafish will be used as experimental models. Based on the knowledge acquired on the new gonadal factors, we propose to employ somatic gene transfer to delay the start of puberty in fish. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA COSTA, DANIEL FERNANDES; RIBEIRO, AMANDA DE OLIVEIRA; RICCI, JULIANA MORENA BONITA; RODRIGUES, MAIRA DA SILVA; DE OLIVEIRA, MARCOS ANTONIO; DA ROSA, IVANA FELIPE; DORETTO, LUCAS BENITES; NAKAJIMA, RAFAEL TAKAHIRO; NOBREGA, RAFAEL HENRIQUE. A83-01 and DMH1 effects in the zebrafish spermatogonial niche: Unraveling the roles of TGF-β and BMP signaling in the Fsh-mediated spermatogonial fate. Gene, v. 897, p. 12-pg., 2023-12-21. (18/16595-7, 22/10421-2, 17/15793-7, 20/15237-0, 19/05643-3, 22/10389-1, 14/25313-4, 21/06742-5)