Effect of a novel nanoemulsion loaded with hesperetin associated with electrical stimulation in bone metabolism inder inflammatory conditions

• Grant number: 22/09690-9
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date until: January 01, 2023
• End date until: June 30, 2023
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Denise Madalena Palomari Spolidorio
• Grantee: Maria Júlia Mancim Imbriani
• Supervisor: Mahmoud Rouabhia
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: Université Laval, Canada
• Associated to the scholarship: 20/14155-0 - Hesperetin-loaded nanoemulsion: Development, characterization and evaluation of its anti-inflammatory and pro-osteogenic, BP.MS

Abstract

New alternative therapies involving natural products and nanobiotechnology open new perspectives to treatment of different diseases. Hesperetin (HT) is a flavonoid, originated from citrus fruits and, presents diverse properties, like antioxidant, anti-inflammatory, analgesic, and benefic effects on bone metabolism that permit its application in nanobiotechnology. The nanoemulsions (NE) are nanoparticle systems capable to control the release and delivery of drugs to a specific site of action, increasing their pharmacokinetic properties. On the other hand, electrical stimulation (ES) has been shown promising effects on cell behavior, presenting anti-inflammatory effects and enhancing bone metabolism functions. In this way, we consider that an association between a nanoemulsion loaded with hesperetin (NE-HT) and electrical stimulation provides the possibility that both can enhance the effects of each other. Therefore, the objective of the present study is to evaluate the effect of a nanoemulsion loaded with hesperetin associated with electrical stimulation on bone metabolism and inflammation. Initially, osteoblasts will be treated with non-cytotoxic concentrations and intensities of NE-HT and ES and stimulated or not by Porphyromonas gingivalis LPS, then their effect on bone metabolism under inflammatory conditions or not will be determined through the formation of mineralized nodules and alkaline phosphatase activity. In sequence, the anti-inflammatory effect of NE-HT and ES will be evaluated through the analysis of cytokine expression by osteoblasts stimulated with P. gingivalis LPS. The numerical data obtained will be submitted to the specific statistical analysis using the software GraphPad Prism. Statistical analysis will be performed at a significance level of 5% (± = 0.05). (AU)