Advanced search
Start date

Effect of a novel nanoemulsion loaded with hesperetin associated with electrical stimulation in bone metabolism inder inflammatory conditions

Grant number: 22/09690-9
Support Opportunities:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): January 01, 2023
Effective date (End): June 30, 2023
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Denise Madalena Palomari Spolidorio
Grantee:Maria Júlia Mancim Imbriani
Supervisor: Dr Mahmoud Rouabhia
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Research place: Université Laval, Canada  
Associated to the scholarship:20/14155-0 - Nanostructured lipid carrier containing hesperitin: Development, characterization and evaluation of its anti-inflammatory and pro-osteogenic, BP.MS


New alternative therapies involving natural products and nanobiotechnology open new perspectives to treatment of different diseases. Hesperetin (HT) is a flavonoid, originated from citrus fruits and, presents diverse properties, like antioxidant, anti-inflammatory, analgesic, and benefic effects on bone metabolism that permit its application in nanobiotechnology. The nanoemulsions (NE) are nanoparticle systems capable to control the release and delivery of drugs to a specific site of action, increasing their pharmacokinetic properties. On the other hand, electrical stimulation (ES) has been shown promising effects on cell behavior, presenting anti-inflammatory effects and enhancing bone metabolism functions. In this way, we consider that an association between a nanoemulsion loaded with hesperetin (NE-HT) and electrical stimulation provides the possibility that both can enhance the effects of each other. Therefore, the objective of the present study is to evaluate the effect of a nanoemulsion loaded with hesperetin associated with electrical stimulation on bone metabolism and inflammation. Initially, osteoblasts will be treated with non-cytotoxic concentrations and intensities of NE-HT and ES and stimulated or not by Porphyromonas gingivalis LPS, then their effect on bone metabolism under inflammatory conditions or not will be determined through the formation of mineralized nodules and alkaline phosphatase activity. In sequence, the anti-inflammatory effect of NE-HT and ES will be evaluated through the analysis of cytokine expression by osteoblasts stimulated with P. gingivalis LPS. The numerical data obtained will be submitted to the specific statistical analysis using the software GraphPad Prism. Statistical analysis will be performed at a significance level of 5% (± = 0.05).

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items

Please report errors in scientific publications list by writing to: