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Nanostructured lipid carrier containing hesperitin: Development, characterization and evaluation of its anti-inflammatory and pro-osteogenic

Grant number: 20/14155-0
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2021
Effective date (End): July 31, 2023
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal researcher:Denise Madalena Palomari Spolidorio
Grantee:Maria Júlia Mancim Imbriani
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Alternative therapies combining natural products and nanobiotechnologies open new perspectives for controlled drug release. In this context, nanostructured lipid systems (NLS) show promising results due to their structural design and outstanding biological properties. Hesperitin (HT) is a metabolite of hesperidin, found mainly in citrus fruits, with several biological properties such as antioxidant, anti-inflammatory, anticancer, neuroprotective, analgesic and with osteogenic capacity. Due to the therapeutic effects of hesperitin, associated with the promising characteristics of NLS, the objective of the present study is to develop a nanostructured lipid carrier containing hesperitin in order to improve its pharmacodynamic properties and thus obtain a biocompatible and bioactive material. Initially, in study 1, nanostructured lipid systems loaded with hesperitin (NLS-HT) will be synthesized and characterized. In study 2, human osteoblast cell lines will be treated with non-cytotoxic doses of NLS-HT and their effects on osteogenesis will be determined through the expression of genes regulating bone metabolism, formation of mineralized nodules, collagen organization and maturation. In study 3, the action of NLS-HT on the expression of inflammatory mediators and matrix metalloproteinases, in addition to its impact on the NF-kB signaling pathways, will be evaluated in human monocytes/macrophages stimulated by Fusobacterium nucleatum. The numerical data, obtained by applying the laboratory protocols, will be subjected to specific statistical analysis using the GraphPad Prism software, and all tests in this study will be applied with a significance level of 5% (p <0.05).

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