Hesperetin-loaded nanoemulsion: Development, characterization and evaluation of its anti-inflammatory and pro-osteogenic

• Grant number: 20/14155-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date until: August 01, 2021
• End date until: October 31, 2023
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Denise Madalena Palomari Spolidorio
• Grantee: Maria Júlia Mancim Imbriani
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 22/09690-9 - Effect of a novel nanoemulsion loaded with hesperetin associated with electrical stimulation in bone metabolism inder inflammatory conditions, BE.EP.MS

Abstract

Alternative therapies combining natural products and nanobiotechnologies open new perspectives for controlled drug release. In this context, nanoemulsions (NE) show promising results due to their structural design and outstanding biological properties. Hesperetin (HT) is a metabolite of hesperidin, found mainly in citrus fruits, with several biological properties such as antioxidant, anti-inflammatory, anticancer, neuroprotective, analgesic and with osteogenic capacity. Due to the therapeutic effects of hesperitin, associated with the promising characteristics of NE, the objective of the present study is to develop a hesperetin-loaded nanoemulsion in order to improve its pharmacodynamic properties and thus obtain a biocompatible and bioactive material. Initially, in study 1, hesperetin-loaded nanoemulsion (HT-NE) will be synthesized and characterized. In study 2, human osteoblast cell lines will be treated with non-cytotoxic doses of HT-NE and their effects on osteogenesis will be determined through the expression of genes regulating bone metabolism, formation of mineralized nodules, collagen organization and maturation. In study 3, the action of HT-NE on the expression of inflammatory mediators and matrix metalloproteinases, in addition to its impact on the NF-kB signaling pathways, will be evaluated in human monocytes/macrophages stimulated by Fusobacterium nucleatum. The numerical data, obtained by applying the laboratory protocols, will be subjected to specific statistical analysis using the GraphPad Prism software, and all tests in this study will be applied with a significance level of 5% (p <0.05).