Analysis of 46 informative markers of ancestry in aspirin users diagnosed with upper gastrointestinal bleeding.

• Grant number: 22/10878-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2022
• End date: July 31, 2023
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Patricia de Carvalho Mastroianni
• Grantee: Isabele Held Lemos
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Introduction and objective: Genomic ancestry is directly linked to the presence and frequencyof genetic variants in a population. To support one of the objectives of an unprecedented studybeing conducted in the Brazilian population by our research group, Auxílio Regular FAPESP,process: 2017/24193-3, it is intended to know the ancestry-informative markers (AIMs) of usersof aspirin diagnosed with upper gastrointestinal bleeding (UGIB), a serious adverse drugreaction. It is also intended to compare the ethnicity self-declared by the participant with theidentified AIMs. Methodology: An experimental genetic study will be conducted using DNAsamples from 50 aspirin users diagnosed with UGIB that were obtained in a case-control studyconducted at the hospital complex of the Hospital das Clínicas of Faculty of Medicine ofUniversity of São Paulo (USP). The diagnosis of UGIB of non-varicose etiology was definedas the presence of gastric or duodenal ulcer findings; erosions, acute lesions of the gastric orduodenal mucosa; and erosive gastritis or duodenitis (with or without active or recent bleeding),through upper digestive endoscopy or exploratory laparotomy. The analysis of 46 AIMs alreadyproposed and validated will be carried out. The analysis of the markers will be performed intwo steps: 1) polymerase chain reaction (PCR) and 2) capillary electrophoresis (ABI 3130sequencer, AppliedBiosystems, Foster City, USA). Analysis of the fragment analysis results willbe conducted in the Gene Mapper software. Expected results: It is expected to describe thegenomic ancestry of aspirin users diagnosed with UGIB in order to identify the populationstratification of the study group.