Evolutionary history of the rare multiple XY1Y2 sex chromosome system. Satellitome analysis in fish and mammal species

• Grant number: 21/14096-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: February 01, 2023
• End date: January 31, 2026
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Marcelo de Bello Cioffi
• Grantee: Geize Aparecida Deon
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated scholarship(s): 23/15593-9 - Sex-Linked markers and Satellite DNA analysis in metatherian mammal species with focus on the evolution of sex chromosomes, BE.EP.PD

Abstract

The present project aims to integrate cytogenetic and genomic analyzes to investigate evolutionary processes that led to the differentiation of the XX/XY1Y2 sex chromosome system, which is rarer among vertebrates compared to other multiple sex chromosome systems. The isolation and characterization of the satellite DNA library (satellitome) will be carried out in representatives of all vertebrate groups (Fish, Eutherians and Metatherians) that carry this rare XX/XY1Y2 system. To this end, species of fish (the catfish Harttia Carvalhoi) and metatherian mammals (the kangaroo Wallabia bicolor) and eutherians (the bat Carollia perspicillata) were selected as models. Subsequently, comparative analyzes of the chromosomal distribution pattern of these satellites, not only between males and females, but also in congeneric species carrying the XX/XY system, will provide conditions to investigate the processes of chromosomal differentiation that occurred after the initial rearrangements that triggered the origin and differentiation of these systems (molecular cytogenetic analyses). Additionally, satDNA families differentially distributed among the sex chromosomes will be selected to investigate the homogenization and recombination rate of different haplotypes (genomic analysis). The selection of vertebrate groups with different characteristics in terms of the amount of repetitive sequences present in their genomes can clarify different evolutionary scenarios regarding the accumulation/loss of repetitive DNAs that occurred in the specific sex chromosomes (Y1 and Y2). At the same time, the project will promote the integration of a network of national and international researchers, equally interested in the subject to be investigated, enabling its execution and obtaining promising results for cytogenetics and evolutionary genetics.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DEON, GEIZE APARECIDA; DOS SANTOS, RODRIGO ZENI; SASSI, FRANCISCO DE MENEZES CAVALCANTE; MOREIRA-FILHO, ORLANDO; VICARI, MARCELO RICARDO; PORTO-FORESTI, FABIO; UTSUNOMIA, RICARDO; CIOFFI, MARCELO DE BELLO. The role of satellite DNAs in the chromosomal rearrangements and the evolution of the rare XY₁Y₂ sex system in Harttia (Siluriformes: Loricariidae). JOURNAL OF HEREDITY, v. 115, n. 5, p. 11-pg., 2024-05-17. (23/00955-2, 22/13381-1, 21/14096-6)