Origin and differentiation of the multiple X1X2Y sex chromosome system: Satellitome analysis in fish, reptile, and mammal species.

• Grant number: 22/00427-3
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: April 01, 2023
• End date: March 31, 2028
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Marcelo de Bello Cioffi
• Grantee: Gustavo Akira Toma
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

The present project aims to integrate cytogenetic and genomic analyzes to investigate the origin and differentiation of the multiple @X1X1X2X2/BX1X2Y sex chromosome system. The isolation and characterization of the satellite DNA library (satellitome) from different vertebrate groups (fish, reptiles and mammals) will be carried out. To this end, fish (the trahira Hoplias malabaricus and the jeju Erythrinus erythrinus), reptile (the legless lizard, Lialis burtonis) and mammal (the night monkey, Aotus infulatus) species were selected as models. Subsequently, comparative analyzes of the chromosomal distribution pattern of these satellites, not only between males and females, but also in congeneric species carrying the @XX/BXY system, will provide conditions to investigate the processes of chromosomal differentiation that occurred after the initial rearrangements that triggered the multiple systems, thus making it possible to advance in the knowledge of their origin and differentiation. The selection of different vertebrate models that have different genomic characteristics regarding the amount of repetitive sequences present in their genomes can clarify distinct evolutionary scenarios regarding the accumulation/loss of repetitive DNAs in the specific sex chromosome (neo-Y). At the same time, the project will foster the integration of a network of national and international researchers, equally interested in the subject to be investigated, enabling its execution and obtaining promising results for cytogenetics and evolutionary genetics. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA, FERNANDO HENRIQUE SANTOS; TOMA, GUSTAVO AKIRA; VIDAL, JHON ALEX DZIECHCIARZ; GARRIDO-RAMOS, MANUEL ANGEL; SOUZA, GUILHERME MOTA; DOS SANTOS, RODRIGO ZENI; PORTO-FORESTI, FABIO; LIEHR, THOMAS; UTSUNOMIA, RICARDO; CIOFFI, MARCELO DE BELLO. Comparative Satellitomics in Arowanas (Telostei, Osteoglossiformes) Sheds Light on the Evolution of Ancient Satellite DNAs. Integrative Zoology, v. N/A, p. 13-pg., 2025-07-17. (22/00427-3, 23/00955-2, 21/11169-2)

TOMA, GUSTAVO AKIRA; SEMBER, ALEXANDR; GOES, CAIO AUGUSTO GOMES; KRETSCHMER, RAFAEL; PORTO-FORESTI, FABIO; BERTOLLO, LUIZ ANTONIO CARLOS; LIEHR, THOMAS; UTSUNOMIA, RICARDO; DE BELLO CIOFFI, MARCELO. Satellite DNAs and the evolution of the multiple X₁X₂Y sex chromosomes in the wolf fish Hoplias malabaricus (Teleostei; Characiformes). SCIENTIFIC REPORTS, v. 14, n. 1, p. 13-pg., 2024-09-02. (23/00955-2, 22/00427-3)

SOUZA, GUILHERME MOTA; KRETSCHMER, RAFAEL; TOMA, GUSTAVO AKIRA; DE OLIVEIRA, ALAN MOURA; DEON, GEIZE APARECIDA; SETTI, PRINCIA GREJO; DOS SANTOS, RODRIGO ZENI; GOES, CAIO AUGUSTO GOMES; GARNERO, ANALIA DEL VALLE; GUNSKI, RICARDO JOSE; et al. Satellitome analysis on the pale-breasted thrush Turdus leucomelas (Passeriformes; Turdidae) uncovers the putative co-evolution of sex chromosomes and satellite DNAs. SCIENTIFIC REPORTS, v. 14, n. 1, p. 14-pg., 2024-09-04. (23/00955-2, 22/14584-3, 22/00427-3)

DE OLIVEIRA, ALAN MOURA; SOUZA, GUILHERME MOTA; TOMA, GUSTAVO AKIRA; DOS SANTOS, NATALIA; SANTOS, RODRIGO ZENI DOS; GOES, CAIO AUGUSTO GOMES; DEON, GEIZE APARECIDA; SETTI, PRINCIA GREJO; PORTO-FORESTI, FABIO; UTSUNOMIA, RICARDO; et al. Satellite DNAs, heterochromatin, and sex chromosomes of the wattled jacana (Charadriiformes; Jacanidae): a species with highly rearranged karyotype. GENOME, v. 67, n. 4, p. 10-pg., 2024-02-05. (22/14585-0, 22/00427-3)