Cytogenomics analysis in the multiple X1X2Y sex chromosomes of the Burton legless lizard Lialis burtonis (Pygopodidae; Squamata): A Comprehensive Approach Using Multi-Genetic Tools.

• Grant number: 24/16036-9
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• Start date: August 01, 2025
• End date: March 31, 2026
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Marcelo de Bello Cioffi
• Grantee: Gustavo Akira Toma
• Supervisor: Tariq Ezaz
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Institution abroad: University of Canberra (UC), Australia
• Associated to the scholarship: 22/00427-3 - Origin and differentiation of the multiple X1X2Y sex chromosome system: Satellitome analysis in fish, reptile, and mammal species., BP.DD

Abstract

This proposal will integrate cytogenetic and genomic approaches to explore the chromosomal rearrangements and the evolution of the multiple ¿X1X1X2X2/¿X1X2Y sex chromosome system in the Australian lizard species: Lialis burtonis (Pygopodidae; Squamata). Besides, we will also analyze a closely related species, Aprasia parapulchella with homologous XY sex chromosomes. We plan to collect these species, isolate and compare their satellitomes to examine their chromosome location and probe for sex-biased satDNA that may be accumulated in their sex chromosomes. Furthermore, we will in situ map eight BAC probes containing sex determining genes of Pogona vitticeps (Agamidae, Squamata) to analyze if these sex-specific sequences are shared between distant related reptiles, and to construct linkage maps of these sex chromosomes. These comparisons, not only between males and females, but also in congeneric species, will allow a thorough inspection of satDNA and sex determining linkage groups that may have taken part in the evolution of these multiple sex chromosomes, expanding the comprehension of the evolution of multiple sex chromosomes in lizards. Furthermore, it will be a unique opportunity for the student to train under a highly renowned research group, expanding his knowledge on robust molecular and cytogenomics techniques.