DOES JABUTICABA HYDROALCOHOLIC EXTRACT HAVE ANTIMICROBIAL ACTION AGAINST CLINICAL MULTIRRESISTANT STRAINS OF Acinetobacter baumannii?

• Grant number: 22/16855-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2023
• End date: February 29, 2024
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Luciane Dias de Oliveira
• Grantee: Sthéfani de Oliveira Dias
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Acinetobacter baumannii is one of the biggest concerns within Intensive Care Units (ICUs), mainly because it has the ability to form biofilms and resist the action of different antibiotics. Therefore, it is extremely important to evaluate the antibacterial action of herbal medicines as a treatment option for these infections. In the present study, the antibacterial and antibiofilm action of the extract of jabuticaba (Myrciaria cauliflora) on three multiresistant clinical strains and a standard strain of Acinetobacter baumannii will be evaluated, in addition, an analysis of the antioxidant and phytochemical activity (flavonoids, phenols and liquid chromatography) will be performed. -HPLC) for identification of extract components. For that, the jabuticaba extract will be prepared and its soluble solids content will be quantified. In the analysis by high performance liquid chromatography (HPLC), an analytical method used in the HPLC equipment will be used, and the determination of the antioxidant action by the 2,2'-diphenyl-1-picrylhydrazyl radical reduction assay (DPPH). The determination of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) will be in accordance with the microdilution test in broth recommended by CLSI M7- A9. Subsequently, the antibiofilm action of the extract will be analyzed using the predetermined minimum bactericidal concentrations (MBC) both to inhibit the formation of the biofilm (prevention) and to treat the formed biofilm, and its viability will be quantified by the MTT test. The data will be analyzed by Graphpad prism 5.0 software with the ANOVA test complemented by the Turkey test with 5% significance (p<0.05).Keywords: