IMMOBILIZATION OF INVERTASE FROM Saccharomyces cerevisiae ON POLY LACTIC ACID (PLA) AND ACRYLONITRILE BUTADIENE STYRENE (ABS) SUPPORTS OBTAINED IN 3D PRINTER

• Grant number: 22/15460-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: May 01, 2023
• End date: April 30, 2024
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Ariela Veloso de Paula
• Grantee: Beatriz Bortoloti Teixeira
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Invertases are enzymes that catalyze the hydrolysis of sucrose into D-glucose and D-fructose, known as invert sugar, which is widely used in the food and beverage industry due to its high sweetness. Because enzymes are one of the most expensive items in a bioprocess, their recovery and reuse is of paramount importance, therefore, immobilizing an enzyme makes the reaction more economically viable. One of the most used principles for immobilization is by covalent bonding, in which there is a connection between active groups of the support and functional groups of the enzyme, resulting in an irreversible immobilization. In this context, additive manufacturing is a new technology that has been gaining more and more space in the industry, with enzymatic immobilization on supports obtained in 3D printer being a promising area with great potential. The thermoplastics most used in 3D printing are poly lactic acid (PLA) and acrylonitrile butadiene styrene (ABS), due to their sustainable, degradable, biocompatible and environmentally friendly nature. However, due to technological innovation, there are still few methodologies, ideal reaction conditions and results reported in the literature and there are no studies on immobilized invertases in PLA and ABS. Therefore, the objective of this project is the immobilization of invertase from Saccharomyces cerevisiae on PLA and ABS supports obtained in 3D printer to prove the innovative potential of this methodology for bioprocesses.