Generation and characterization of different liquids activated with plasma for use in endodontic treatment

• Grant number: 23/02268-2
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 2023
• End date: September 30, 2025
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Rodrigo Savio Pessoa
• Grantee: Nilton Francelosi Azevedo Neto
• Host Institution: Divisão de Ciências Fundamentais (IEF). Instituto Tecnológico de Aeronáutica (ITA). Ministério da Defesa (Brasil). São José dos Campos , SP, Brazil
• Associated research grant: 19/05856-7 - Use of low temperature atmospheric pressure plasma in dentistry: from laboratory bench to clinics, AP.TEM

Abstract

This project aims to investigate the generation of Plasma-Activated Liquid (PAL) for dentistry applications. Due to its promising potential, there is a pressing need to develop a pilot-scale PAL system that can generate tangible results in the field of dentistry. This postdoctoral project will concentrate on: (i) developing and characterizing a system to generate various types of PAL, and (ii) applying these plasma-activated liquids to endodontic treatments. Techniques such as mass spectrometry and optical emission spectroscopy will be utilized to study the physical and chemical properties of the generated plasmas and their interaction with PAL. In microbiological tests, argon/air plasmas will be applied to physiological solution, deionized water, and distilled water and their antimicrobial activity against dual-species biofilms of Candida albicans (SC 5314) and Enterococcus faecalis (ATCC 29212) will be evaluated. Upon determining the optimal conditions for biofilm inactivation, experiments will be conducted where the dual-species biofilms will be grown in the root canal of 50 single-rooted bovine teeth fragments and treated with PAL. Through microbiological and plasma-treated liquid analyses, the actions of PAL on microorganisms will be explored and a protocol for its use in dentistry will be established. Lastly, a PAL generation system will be devised for use in dental clinics.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NETO, NILTON F. AZEVEDO; MIRANDA, FELIPE S.; MOREIRA JR, PEDRO W. P.; GOMES, MARCELO P.; ALVES JR, CLODOMIRO; KOGA-ITO, CRISTIANE Y.; PESSOA, RODRIGO S.. Physical and chemical characteristics of plasma-activated water generated by hybrid dielectric barrier discharge and gliding arc discharge. JOURNAL OF PHYSICS D-APPLIED PHYSICS, v. 57, n. 41, p. 15-pg., 2024-10-18. (19/05856-7, 21/14181-3, 23/02268-2)

SACCOMAN, JOAO; NETO, NILTON FRANCELOSI A.; DA SILVA, JOSE H. D.. Deposition rate and optical emissions in niobium oxide processes by reactive sputtering. JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A, v. 42, n. 3, p. 6-pg., 2024-05-01. (20/12356-8, 23/02268-2)

KOGA-ITO, CRISTIANE YUMI; KOSTOV, K. G.; MIRANDA, F. S.; MILHAN, N. V. M.; NETO, N. F. AZEVEDO; NASCIMENTO, F.; PESSOA, R. S.. Cold Atmospheric Plasma as a Therapeutic Tool in Medicine and Dentistry. PLASMA CHEMISTRY AND PLASMA PROCESSING, v. N/A, p. 37-pg., 2023-08-27. (16/07196-6, 21/14181-3, 23/02268-2, 19/05856-7, 20/09481-5)

AZEVEDO NETO, NILTON F.; CALLIGARIS, GUILHERME A.; AFFONCO, LUCAS J.; ZANATTA, ANTONIO R.; SOARES, MARCIO M.; DA SILVA, JOSE H. D.. The role of the substrate on the structure of reactive sputtered Co3O4: From polycrystalline to highly oriented films. Thin Solid Films, v. 782, p. 7-pg., 2023-09-07. (20/12356-8, 23/02268-2, 17/18916-2)

NETO, NILTON F. AZEVEDO; MARQUES, SAMUEL A.; MIRANDA, FELIPE S.; JUNIOR, PEDRO W. P. MOREIRA; PEREIRA, ANDRE L. J.; CONSTANTINO, CARLOS J. L.; DA SILVA, JOSE H. DIAS; PESSOA, RODRIGO S.. Sputter deposited silver film as an alternative tool for Raman signal enhancement in plasma-activated water. VIBRATIONAL SPECTROSCOPY, v. 136, p. 8-pg., 2024-12-19. (22/11544-0, 23/02268-2, 21/14181-3, 19/05856-7, 18/22214-6, 23/02273-6)

NETO, NILTON F. AZEVEDO; MIRANDA, FELIPE S.; MOREIRA JR, PEDRO W. P.; GOMES, MARCELO P.; ALVES-JUNIOR, CLODORIMO; KOGA-ITO, CRISTIANE Y.; PESSOA, RODRIGO S.. Serial association of dielectric barrier discharge and gliding arc systems: a novel approach for plasma-activated saline production. JOURNAL OF PHYSICS D-APPLIED PHYSICS, v. 58, n. 1, p. 12-pg., 2025-01-06. (23/02268-2, 21/14181-3, 19/05856-7)