Mapping of polysaccharide distribution on sugar cane ceell wall using Carbohydrate Binding Modules labeled with extrinsic probes

• Grant number: 23/03700-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 2023
• End date: October 31, 2025
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Agreement: CNPq - INCTs
• Principal Investigator: Marcos Silveira Buckeridge
• Grantee: Carolina Victal Garbelotti
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 14/50884-5 - INCT 2014: National Institute of Science and Technology of Bioethanol, AP.TEM
• Associated scholarship(s): 23/17357-0 - Mapping polysaccharide distribution in sugarcane cell wall using Carbohydrate Binding Modules labelled with extrinsic probes and secondary ion mass spectrometry imaging., BE.EP.PD

Abstract

Plant cell walls are an important renewable raw material for the sustainable generation of fuels, materials and chemicals. The recalcitrance of the complex network of polysaccharides and lignin that make up the lignocellulosic component of plant cell walls makes it difficult to deconstruct the biomass by hydrolytic enzymes. Detailed knowledge of cell wall architecture can provide a rationale for improving enzymatic degradation by targeting specific polysaccharides. As a proof of principle, we propose to study the effect of enzymatic treatment of plant cell walls using cell surface sensitive techniques to follow the effect of enzymatic activity on cell wall architecture. The effect of hydrolytic enzyme treatments on polysaccharide content and distribution in sugarcane stem sections will be evaluated by monitoring the binding of extrinsically labeled carbohydrate binding modules using images generated by fluorescence lifetime imaging microscopy (FLIM) along with advanced secondary ion mass spectrometry (SIMS) based imaging.