Investigation of acetaldehyde formation via pyruvate dehydrogenase reaction

• Grant number: 23/06352-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2023
• End date: July 16, 2024
• Field of knowledge: Biological Sciences - Biochemistry - Metabolism and Bioenergetics
• Principal Investigator: Marisa Helena Gennari de Medeiros
• Grantee: Rafael Andrade Rebelo
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 13/07937-8 - Redoxome - Redox Processes in Biomedicine, AP.CEPID

Abstract

Acetaldehyde produced endogenously or by exogenous exposure is associated with several pathophysiological processes. Accumulation of endogenous aldehydes leads to genotoxicity. Aldehydes react with amine and thiol groups of proteins, nucleic acids and some lipids and promote changes in these biomolecules. Recently, a pathway for the production of acetaldehyde via ketoacid dehydrogenases, especially pyruvate dehydrogenase, has been proposed in the literature. However, the biological conditions for this generation are still unclear. This project aims to monitor the formation of isotopically labeled acetaldehyde by mass spectrometry in different cell lines and under different stress conditions, such as hypoxia in cells supplemented with [13C6]-glucose. This project is part of studies being carried out by CEPID-Redoxoma.