Two different analysis method: table-top and hand-held optical reflectometer in the in vitro early erosion study

• Grant number: 23/04757-0
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: July 16, 2023
• End date: August 30, 2023
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Daniela Rios Honório
• Grantee: Thayna Teodoro da Silva
• Supervisor: Thiago Saads Carvalho
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Institution abroad: University of Bern, Switzerland
• Associated to the scholarship: 21/10381-8 - Is the association between proanthocyanidin and palm oil able to reduce the initial erosion enamel?, BP.IC

Abstract

This in vitro study will evaluate the effectiveness of Proanthocyanidin and Palm Oil isolated and associated in preventing initial erosion. Bovine teeth will be divided into 6groups (n = 6): G1- Elmex erosion protection (positive control), G2- Deionized water (negative control), G3- 100% palm oil, G4- 2% Proanthocyanidin, G5- 100% palm oil + 2% Proanthocyanidin, G6- 2% Proanthocyanidin + 100% Palm Oil. The acquired enamel pellicle will be formed in vitro by using a previously collected saliva. The treatment will be carried out by applying the solutions for 30 seconds. Soon after, the enamel blocks will be submitted to demineralization by citric acid (0.5%, pH 2.5) for 30 seconds. Then, they will be rinsed in deionized water to cease the demineralization process for measuring erosive demineralization. The response variable will be made with the analysis of surface reflection intensity of the specimens by using a reflectometer (Hand-held and Table-top reflectometer). Data will be checked for normality and homogeneity. If they pass these tests, two-way ANOVA will be obtained followed by Tukey's test (p <0.05). (AU)