SENSITIVITY OF CYTOPATHOLOGICAL EXAMINATION FOR THE DIAGNOSIS OF OSTEOSARCOMA IN DOGS

• Grant number: 23/03948-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2023
• End date: July 31, 2024
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Noeme Sousa Rocha
• Grantee: Gabriela de Almeida Fernandes e Silva
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The increase in life expectancy, consequent of social, medical and technological advances in a short period of time, led to the expressive increase of cancers development among humans and also among animals, specially dogs. In this regard, it is necessary to improve the knowledge in the oncology area and understand your clinical applicability. In the case of osteosarcoma (OSA), the dog has been suggested as a research model for the disease, because even though it is rare in humans, it is relatively common in the canine species; besides having similarities in it natural history. The OSA diagnosis, yet, faces challenges; the histopathology is considered comprehensive for being based on tissue architecture, however, presents negative points: high cost and more time for processing specially for the bone, due to decalcification; when compared to the fine needle aspiration cytology (FNAC) technique. That way, this work aims to clarify the role of FNAC in the OSA diagnosis, through the elucidation of the malignant criterias found in this technique, looking for identify the exclusive and concurrent microscopic aspects of cytological and histopathological exams. Furthermore, it will be made an evaluation of tumor aggressiveness through immunohistochemical markers, being used osteocalcin and Ki-67, in order to check the accuracy achieved by FNAC. Thereunto, it will be made a retrospective study with canine OSA diagnosed, from 2017 to 2022, at the Veterinary Hospital and Veterinary Pathology Service FMVZ - UNESP Botucatu Campus. This analysis will be important for the understanding of cytology in the diagnosis of canine OSA, aiming into turning it a viable, safe and reliable choice, that comprehends both veterinary and human medicine and supports patients prognosis and treatments.