Optimization of B. subtillis KO7 fermentation using low-cost substrates in high-throughput microscale bioreactors

• Grant number: 23/06171-3
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: August 01, 2023
• End date: November 30, 2023
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Danielle Biscaro Pedrolli
• Grantee: Lucas Moita Paim
• Supervisor: Leonardo Rios Solis
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Institution abroad: University College London (UCL), England
• Associated to the scholarship: 22/12656-7 - DEVELOPMENT OF LOW-COST EXPRESSION SYSTEMS FOR THE PRODUCTION OF T5 EXONUCLEASE, BP.IC

Abstract

One of the main goals of a bioprocess is to gather as much information as possible to allow the development and production of the bioproduct with high yields since the viability of using synthetic systems in an industrial setting depends mostly on the costs of the bioprocess and the yield of the systems. However, traditional methods are still costly, and labour-intensive, and the number of experiments that can be performed simultaneously are limited. To save costs and time in process development, miniaturized bioreactors are used as an alternative to bench-scale bioreactors the traditional approach. Today the most recent technics involve High-throughput systems that can run 48 fully controlled and scalable cultivations in a deep-well microtiter plate, and allow oxygen transfer rates similar to bench-top bioreactor systems. However, are few studies that aim to optimize heterologous expression using microreactors and non-point-to-point optimization technics. In this sense, this project purpose optimizes the cultivation of B. subtillis KO7 using low-cost culture media and substrates by the use of High-throughput microreactors systems combined with experimental design and response surface methodology. Thus, this project foreseen identify the optimized conditions and the influence of supplementing different carbon sources (glucose, xylose, glycerol and sucrose) in Luria Bertani medium on the cultivation of B. subtilis KO7 in the proposed time period, which would be impractical with traditional techniques, in this project or in the current country project schedule. (AU)