Development of protocols for conservation and culture of cells to obtain cytogenetic preparations in Cervids

• Grant number: 23/07473-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2023
• End date: June 30, 2026
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: José Maurício Barbanti Duarte
• Grantee: Eluzai Dinai Pinto Sandoval
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated research grant: 17/07014-8 - The use of actual topotypes to produce genotypes and cytotypes in the taxonomic review of the Mazama genus: the basis for the species conservation, AP.BTA.TEM
• Associated scholarship(s): 24/04621-4 - Collection, long-term preservation, and in vitro culture of sheep testis as model species for endangered deer species, BE.EP.PD

Abstract

The genus Mazama groups Neotropical deer of medium to small size and simple antlers in males. The morphological similarity between the species of the genus has been described, contrasting with the karyotypic variation between them. Thus, the study of chromosomes is an efficient tool assisting the species delimitation in which karyotypical divergences may cause reproductive isolation. However, obtaining chromosomal preparations from the specimens requires sampling collection, mainly skin and blood, that after slow freezing process and tissue culture, allow cell multiplication for use in classical and molecular cytogenetic techniques. Simplifying the tissue storage process is an important step to access material from specimens collected in other countries with less technical conditions. Thus, it will allow accessing material from individuals that may be collected by local partners in different locations in Latin America and,in that way, expand the sampling in places where displacement with large equipment and teams is limited. This project aims to enable simpler, cheaper and viable transport and storage methods from tissue collected from deer, specifically of the genus Mazama. Therefore, assays will be performed to establish simplified media for skin tissue freezing, storage and culture. These new techniques should allow greater ease and viability for cytogenetic studies of Cervidae, adding sampling samples to the thematic project to which this grant is linked.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANDOVAL, ELUZAI DINAI PINTO; DUARTE, JOSE MAURICIO BARBANTI. Transport Media for Live Skin Tissue from Gray-Brocket Deer (Subulo gouazoubira). BIOPRESERVATION AND BIOBANKING, v. N/A, p. 7-pg., 2025-03-27. (17/07014-8, 23/07473-3)