Development of a hybrid nanostructure for the antibiofilm action

• Grant number: 23/07672-6
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• Start date: September 25, 2023
• End date: April 05, 2024
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Ana Olívia de Souza
• Grantee: Luiz Gustavo Ribeiro
• Supervisor: Juan Paulo Hinestroza
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Institution abroad: Cornell University, United States
• Associated to the scholarship: 20/03883-4 - Study of biological silver nanoparticles effect on Zebrafish, Nile Tilapia and Lambari, BP.DD

Abstract

Nanoparticles, such as silver nanoparticles (AgNPs), are one of promising candidates for biomedical and pharmaceutical use. Mycogenic AgNPs, coded as AgNP-AT, were previously obtained extracellularly using the fungus Aspergillus tubingensis, and characterized physical and chemically via size measurement, polydispersity index (PDI), zeta potential through dynamic light scattering (DLS), transmission electron microscopy (TEM), and Fourier transform infrared (FTIR) spectroscopy. Another kind of nanomaterial of interest for biomedical and pharmaceutical applications is a nanostructured hydrogel biocompatible and biodegradable. Cellulose nanofibers (CNFs), renewable and recyclable polymer obtained from natural sources, are commonly used in hydrogel preparation. CNF-based hydrogels can be combined with nanomaterials to enhance their wound healing properties. The main aim of this study is to associate the CNF-based hydrogels with AgNP-AT to improve interaction of these two compounds with biological systems and potentially used this biocomoposite as antibiofilm agent. The study will be use the TEMPO oxidation technique to prepare hydrogel films and aerogels that will incorporate AgNP-AT. The hybrid nanostructured material will be characterized using UV-Vis, ATR-FTIR spectrometry, field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), rheometry and water retention properties. (AU)