Prospection of Aspergillus niger genes for the production of new enantioselective lipases of industrial interest

• Grant number: 22/15643-3
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: September 01, 2023
• End date: October 06, 2027
• Field of knowledge: Biological Sciences - Biochemistry - Enzymology
• Principal Investigator: Patrícia de Oliveira Carvalho
• Grantee: Pedro Henrique Dias Garcia
• Host Institution: Universidade São Francisco (USF). Campus Bragança Paulista. Bragança Paulista , SP, Brazil

Abstract

In the present project we propose conducting an unprecedented study based on rational engineering of the lipase-producing species Aspergillus niger, previously studied by our group, with a view to developing new, highly enantioselective enzymes. The genome of Aspergillus includes many genes of enzymes that have yet to be investigated and therefore potentially concealing new lipases to be used by industry. The means to be employed include bioprospection for genes and the production of recombinant lipases using the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) technique, purification of new enzymes, and their posterior application in enantioselectivity models with racemic molecules. The project can be expected to expand the knowledge and selection of new enzymes with improved biochemical and kinetic properties as potential catalysts for the fine chemicals industry. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA, ALEX A. R.; COELHO, LILIA L. D.; SANCHEZ-LUQUEZ, KAREN Y.; GARCIA, PEDRO H. D.; BOLDIN, RENATA; SILVEIRA, ANNE M. R.; ANTONIO, MARCIA A.; PORCARI, ANDREIA M.; CARVALHO, PATRICIA O.. Determination of Lisdexamfetamine in Human Plasma by LC-MS/MS Method. Journal of the Brazilian Chemical Society, v. 36, n. 4, p. 8-pg., 2025-01-01. (22/04341-6, 22/15643-3)