Analysis of histopathological effects and endocrine disruption in chronic exposure to Diflubenzuron and Piriproxyfen in the experimental model Danio rerio

• Grant number: 23/09884-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2023
• End date: August 31, 2027
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Bruno Fiorelini Pereira
• Grantee: Rafaella da Silva Brito
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated research grant: 22/03094-5 - Project Zebratox: toxicological screening of larvicides of public and veterinary health importance, AP.PNGP.PI
• Associated scholarship(s): 25/07127-3 - Application of Biomarkers for Endocrine Disruptor Screening in Zebrafish: A Multi-Endpoint Approach to Assessing the Effects of Diflubenzuron and Pyriproxyfen, BE.EP.DR

Abstract

The anthropic impacts on aquatic environments lead to the exposure of fauna and flora to numerous substances, whose real effects are often unknown. Among the agents that are constantly released, pesticides are relevant, not only because of the amounts used, but also because of the diversity of compounds produced and registered in Brazil. Although some experts defend that there is no risk if the amount of active ingredients is within the regulated limit, data on the occurrence of active ingredients of pesticides in Brazilian aquatic environments are still scarce. Among ecotoxicological tests with fish, the zebrafish-based model has become popular for assessing toxicity, mainly because it is an organism with anatomy, physiology and development similar to higher vertebrates and because it is representative and recognized as a model in aquatic environments. Therefore, this project aims to use several biomarkers to evaluate the effects of the active ingredients of the compounds Diclubenzuron (DBZ) and Pyriproxyfen (PPF), two widely used pesticides that may pose risks to aquatic life and human health, focusing on their individual action. For this, this project will carry out analyzes aimed at identifying histopathological changes in the liver, gills and gonads, in addition to the possible action of these agents as endocrine disruptors. These analyzes bring a multidisciplinary character to the project and will allow the production of relevant data for the growth of knowledge of environmental toxicology and the identification of how such substances in a mixture can interact with the biota, aiming at the conservation of species and the generation of data that help to reformulate and update Brazilian legislation. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TOMINAGA, FLAVIO KIYOSHI; BRITO, RAFAELLA SILVA; DOS SANTOS, ISIS WANESSA FERREIRA; VALLE, AMANDA DE AZEVEDO; GIANNOCCO, GISELE; MACIEL, RUI MONTEIRO DE BARROS; KUMMROW, FABIO; SOUZA, MARILIA CRISTINA OLIVEIRA; LUCHIARI, ANA CAROLINA; PEREIRA, BRUNO FIORELINI. Microplastic Interference with Fipronil Toxicity to Zebrafish Embryonic Development. MICROPLASTICS, v. 3, n. 4, p. 13-pg., 2024-12-01. (23/09884-0, 24/01418-3, 22/03094-5, 24/10993-1, 23/15540-2)

TOMINAGA, FLAVIO KIYOSHI; BRITO, RAFAELLA SILVA; DO NASCIMENTO, JULIANA OLIVEIRA; GIANNOCCO, GISELE; MACIEL, RUI MONTEIRO DE BARROS; KUMMROW, FABIO; PEREIRA, BRUNO FIORELINI. Pyriproxyfen toxicity to fish and crustaceans: A literature review. Environmental Research, v. 274, p. 20-pg., 2025-03-07. (23/09884-0, 24/01418-3, 24/10993-1, 22/03094-5)