Phylogenomics, population genomics and identification via CRISPR SHERLOCK of Dyckia's complex of species from the quadrilátero ferrífero of Minas Gerais

• Grant number: 23/00765-9
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: September 01, 2023
• Status: Discontinued
• Field of knowledge: Biological Sciences - Botany - Applied Botany
• Principal Investigator: Maria Imaculada Zucchi
• Grantee: João Victor da Silva Rabelo Araujo
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 25/03280-1 - Chromosomal-Level Genome Assemblies for Endangered Dyckia Species: A Pathway to Understanding Local Adaptation and Conservation, BE.EP.DD

Abstract

Dyckia spp. are xenomorphic bromeliads, whose center of diversity is the Campos Rupestres of Serra do Espinhaço, in the state of Minas Gerais. Despite its great diversity, most of the species in the region are threatened by mining and farming activities. In addition, the low morphological heterogeneity between species and the absence of records in herbariums, makes its identification a challenge for its proper management. We aim to develop in situ and ex situ conservation strategies for the Dyckia complex of Serra do Espinhaço, through understanding its genetic diversity and population structure, including the development of an in vitro culture bank in a protected environment. To do so, we will determine the location and abundance of the remnants of the Dyckia complex, and use the sequencing genotyping technique (GBS) to identify single nucleotide polymorphisms (SNPs), seek to detect loci that may be under the influence of environmental selection, proposing possibilities about the history of dispersal, and depression, of the species. In addition, in a pioneering way, we will develop a rapid identification tool at the molecular level of species to be used in the field through the recently created CRISPR-SHERLOCK technology, thus facilitating the distinction of species without the need for DNA acceptance in the laboratory and streamlining everyday decision-making in the natural environment by all professionals. This project will contribute to the conservation of species threatened by human activities in the Serra do Espinhaço, facilitating decision-making regarding their sustainable management. (AU)