Bioassays and 2D and 3D cell culture techniques to evaluate immune responses of pau-d'arco extracts

• Grant number: 23/13973-9
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: December 15, 2023
• End date: February 14, 2024
• Field of knowledge: Engineering - Chemical Engineering - Industrial Operations and Equipment for Chemical Engineering
• Principal Investigator: Wanderley Pereira Oliveira
• Grantee: Gabriela Alberto Gil
• Supervisor: Mandip Singh Sachdeva
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Florida Agricultural and Mechanical University (FAMU), United States
• Associated to the scholarship: 23/01007-0 - Technological development and evaluation of the biological activity of phytoactives from Handroanthus impetiginosus (Mart. ex DC.) Mattos, BP.IC

Abstract

Medications derived from plants used in traditional medicine represent an important source of new therapeutic agents for numerous diseases. Handroanthus impetiginosus (Mart. ex DC.) Mattos (Bignoniaceae Juss Family), commonly known as pau-d'arco, possesses immense biological potential and has a historical track record in medicine for treating skin cancer and autoimmune diseases. However, despite these attributes, studies focused on standardization with the qualitative and quantitative characterization of their main metabolites, as well as the validation of their biological activity, bioavailability and toxicity are relatively scarce. In this context, this project seeks to provide the scientific initiation student with expertise in 2D and 3D cell culture techniques, focusing on evaluating the immune responses elicited by cell cultures exposed to pau-d'arco extracts. (AU)