Standardization and application of eggshell membrane in the healing of extra and intra oral wounds in type 1 diabetes

• Grant number: 23/13506-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: January 01, 2024
• End date: December 31, 2026
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Mario Taba Junior
• Grantee: Paola Gomes Souza
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Healing is a process characterized by sequential stages of hemostasis, inflammation, proliferation and maturation, for which biomaterials have been used with the aim of making it viable. Among these, the exploration and development of a new natural and sustainable resource stands out, the chicken eggshell membrane (EMS), a versatile and abundant biomaterial with excellent physical, chemical, mechanical and biological properties. It is known that EMS has the ability to promote progenitor cell dynamics and accelerated cellular differentiation, due to the enhancement, which induces the properties of vascularization and angiogenesis, the controlled inflammatory process and the stimulation of protease activity. However, the effect of EMS on the healing of extra- and intra-oral wounds concomitant with type 1 diabetes has not yet been elucidated in the literature. Our hypothesis is that EMS can positively interfere with the healing process associated with its enrichment with hyaluronic acid and, therefore, the objective of this project is to evaluate the effect of EMS on the healing of extra and intra oral wounds in type 1 diabetes. Protocols obtaining and processing EMS will be tested and characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, fluid absorption and chorioallantoic membrane tests. The concentration of hyaluronic acid with the greatest effects on fibroblasts cultured under EMS will be determined by evaluating cell adhesion, viability, proliferation and migration in vitro. And the effect of using EMS associated with hyaluronic acid on extra and intra oral wounds in type 1 diabetes in vivo will be determined by (1) wound monitoring through photographic records, (2) histological analysis, (3) gene expression through PCR in real time and (4) proteins involved in healing by Western blot. The results of this study may contribute to the establishment of new therapeutic approaches that are viable for everyday clinical practice, favoring an alternative that positively induces the healing process of wounds on the skin and mucosa or to be used as a dressing for postoperative protection after oral surgeries in patients with type 1 diabetes.