Development of a scaffold-on-a-chip platform using calcium phosphate coated-ZrO2 scaffolds obtained by flash sintering

• Grant number: 23/15038-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: July 01, 2024
• End date: June 30, 2025
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Principal Investigator: Eliria Maria de Jesus Agnolon Pallone
• Grantee: Fabio Caixeta Nunes
• Supervisor: Yu Shrike Zhang
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Institution abroad: Harvard University, Boston, United States
• Associated to the scholarship: 22/05031-0 - Flash sintering of ZrO2 scaffolds obtained by 3D printing for tissue bioengineering, BP.DR

Abstract

Zirconia (ZrO2) scaffolds are widely used in hard tissue engineering primarily due to their intrinsic properties, such as hardness, wear resistance, and aesthetics. For bone regeneration, the surface of these scaffolds can be modified to improve their surface properties and achieve bioactivity, for instance, using calcium phosphate (CaP) coatings. These coated scaffolds can be combined with microfluidic devices, known as organ-on-a-chip, to create in vitro models that recapitulate the microenvironment of organs and tissues. In this context, this work aims to conduct in vitro tests on ZrO2 scaffolds obtained through extrusion-based 3D printing and conventional sintering, as well as through flash sintering (FS). Furthermore, these scaffolds will be tested using in vitro organ modeling to mimic the physiological environment of bone tissue. In Brazil, we developed a printable ink based on polyethyleneglycol and laponite, to which ZrO2 particles were added. The scaffolds were printed with dimensions of 10x10x5 mm, and the debinding process was performed at 800°C/1h. A portion of the scaffolds was conventionally sintered, while another portion was printed using the FS technique, with an electric field of 80 V/cm. The surfaces of the scaffolds were chemically modified. Now, for this second stage of the work at Harvard Medical School, we aim to conduct several in vitro experiments on the scaffolds to thoroughly investigate their interactions with cells. In addition, experiments involving microfluidic technologies that provide biomimetic tissue culture conditions can be performed to apply and validate organ-on-a-chip technologies in bone research using ZrO2.

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

NUNES, FABIO C.; GOMES, GUSTAVO H. M.; CAMPOS, JOAO V.; DAGUANO, JULIANA K. M. B.; PALLONE, ELIRIA M. J. A.. Influence of controlled current ramp on flash sintering of 3D-printed zirconia scaffolds. Materials Letters, v. 398, p. 5-pg., 2025-11-01. (22/05031-0, 21/06509-9, 23/18168-7, 23/15038-5, 22/10604-0)

NAGATA, E. Y.; NUNES, F. C.; SANTANA, L.; SILVEIRA, Z. C.; FERREIRA, J. A.; AMBROSIO, C. E.; PALLONE, E. M. J. A.. Strontium-enriched phosphate coating on fused filament fabrication 3D printed alumina scaffolds. Materials Letters, v. 399, p. 5-pg., 2025-11-15. (22/05031-0, 22/10604-0, 24/14631-7, 23/15038-5)

NUNES, FABIO C.; LANCONI, PATRYCK A.; GOMES, GUSTAVO H. M.; SANTOS, KARINA F.; NAGATA, ESTER Y.; CAMPOS, JOAO, V; MORAES, IZABEL C. F.; DAGUANO, JULIANA K. M. B.; PALLONE, ELIRIA M. J. A.. Flash sintering of 3D-printed 3YSZ scaffolds for bone tissue engineering. CERAMICS INTERNATIONAL, v. 51, n. 13, p. 14-pg., 2025-05-01. (23/15038-5, 21/06509-9, 22/05031-0, 23/18168-7, 22/10604-0)