Bioengineering of Bifunctional Metalloproteins Applied in the Production of Added Value Products

• Grant number: 23/10667-4
• Support Opportunities: Scholarships in Brazil - Support Program for Fixating Young Doctors
• Start date: August 01, 2023
• End date: November 06, 2024
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Agreement: CNPq
• Principal Investigator: Frank Nelson Crespilho
• Grantee: Rodrigo Michelin Iost
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 23/01529-7 - Bioengineering of bifunctional metalloproteins applied in the production of added value products, AP.R

Abstract

The production of value-added products from enzymes or redox proteins found in nature is limited. This limitation can be overcome in the laboratory by modifying native enzymes, or by obtaining artificial apo-reconstituted proteins. In this project, we intend to contribute to frontier research in the area of metalloprotein bioengineering and to obtain new apo-reconstituted structures. Thus, the aim is to modify the structure of proteins with metallic centers to electrochemically produce value-added organic molecules from the electroreduction of CO2 (ERCO2). Since the active site of the redox protein will be modulated, the bioelectroreduction reaction of the H2O molecule (BRH2O) to produce molecular hydrogen (H2) will also be investigated; bifunctional metalloproteins will be obtained. With the completion of this project, there will be prospects for transferring the methods to be developed to the technology sector. In addition, the work to be developed in the environment of the Institute of Chemistry of São Carlos (IQSC-USP), will contribute both to strengthening the research of the Bioelectrochemistry & Interfaces group and to the know-how of researching apo-reconstituted proteins with a focus on in the production of value-added products.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BERTAGLIA, THIAGO; NEUBERT, TILMANN J.; IOST, RODRIGO M.; BALASUBRAMANIAN, KANNAN; CRESPILHO, FRANK N.. Consequences of edge and substrate modifications on graphene electrochemistry. CURRENT OPINION IN ELECTROCHEMISTRY, v. 50, p. 9-pg., 2025-01-25. (20/12404-2, 22/09164-5, 23/08260-3, 22/06563-6, 18/22214-6, 23/10667-4, 20/03681-2, 23/01529-7, 23/13288-4)

IOST, RODRIGO M.; VENKATKARTHICK, RADHAKRISHNAN; NASCIMENTO, STEFFANE Q.; LIMA, FABIO H. B.; CRESPILHO, FRANK N.. Hydrogen bioelectrogeneration with pH-resilient and oxygen-tolerant cobalt apoenzyme-saccharide. CHEMICAL COMMUNICATIONS, v. 60, n. 18, p. 3-pg., 2024-02-01. (22/09164-5, 23/10667-4, 21/05665-7, 18/22214-6, 23/01529-7, 20/15098-0)