EVALUATION OF CYTOTOXICITY AND PHYSICAL PROPERTIES OF RESIN INFILTRANTS CONTAINING HYDROFOBIC MONOMER

• Grant number: 23/09074-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2024
• End date: February 28, 2026
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Giselle Maria Marchi
• Grantee: Isadora Cezar Rodrigues
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

The objective of this study is to evaluate the color stability, surface roughness, degree of conversion and cytotoxicity of experimental resin infiltrants containing different concentrations of the monomer 1,12 dodecanediol dimethacrylate (DDDMA). The experimental infiltrants will be manipulated with 85% TEGDMA, 15% DDDMA, 1%EDAB and 0.5% CQ (IE15) and 70% TEGDMA, 30% DDDMA, 1%EDAB and 0.5% CQ (IE30). Forty-five blocks of enamel/dentin, from bovine teeth, will be made in dimensions of 5 x 5 x 3 mm. Afterwards, they will be submitted to cycles of demineralization/remineralization (DES-RE) for 7 days, for the formation of the white spot lesion. Then, the enamel surfaces will receive treatments according to the different groups: IC group: Icon ® infiltrant (commercial control), IE15 group: experimental infiltrant with 15% DDDMA and IE30 group: experimental infiltrant with 30% DDDMA. The color stability test (n=15) will be performed with a dye solution (coffee) for 14 days, and the specimens will have their color measured in a VITA Easyshade® spectrophotometer at different times: before immersion (T0), after 7 days of immersion (T1), after 14 days of immersion (T2) and after final polishing (T3), obtaining CIEL*a*b* values for calculating the color change. The surface roughness test (n=15) will be performed in a rugosimeter, following the same times as the color stability test: (T0) before immersion, (T1) after 7 days of immersion; (T2) after 14 days of immersion; (T3) after final polishing; in this way, the mean changes in surface roughness between groups will be compared. For the degree of conversion (n=5), measurements will be taken before and after photoactivation of the samples, using the Fourier Transformed Infrared Ray Spectrometer (FTIR). For cytotoxicity (n=10), infiltrant discs from each group and the fibroblast cell line will be used. Two analyzes will be carried out: direct contact; and after 24 hours. The test will be performed with the MTT solution (3 - (4,5 - dimethyl - 2 - thiazolyl) - 2,5 - diphenyl - 2H - tetrazolium bromide) (1 mg/mL) in polystyrene plates with 96 wells. The absorbance will be measured with the microplate reader and the average between the tested sample and the blanks will express the viability of the groups. The results will be subjected to homogeneity and normality tests to be subsequently analyzed using the most appropriate test, with a significance level of 5%.