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ASSESSMENT OF FUMONISIN REMOVAL BY STRAINS OF LACTIC ACID BACTERIA AND YEASTS

Grant number: 24/03289-6
Support Opportunities:Scholarships abroad - Research Internship - Doctorate (Direct)
Effective date (Start): May 31, 2024
Effective date (End): October 30, 2024
Field of knowledge:Agronomical Sciences - Food Science and Technology
Principal Investigator:Carlos Augusto Fernandes de Oliveira
Grantee:Lucas Gabriel Dionisio Freire
Supervisor: Giuseppina Avantaggiato
Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Research place: Institute Of Sciences Of Food Production, Italy  
Associated to the scholarship:22/05066-9 - Assessment of functional yoghurt efficacy to decrease the toxic effects of aflatoxins alone or in combination with fumonisins on piglets, BP.DD

Abstract

Fumonisins are toxic compounds produced by fungi that greatly impact human and animal health. Biological decontamination methods have been testing the microorganism's ability to adsorb mycotoxins and reduce their toxicity. However, the literature data regarding fumonisin removal is scarce compared to aflatoxin. This research project aims to assess the in vitro removal of fumonisin B1 (FB1) by commercial strains of Lactobacillus rhamnosus (HOWARU® LYO 40 DCU) and Saccharomyces cerevisiae (Fermentis K-97, SafAle, Bruggeman, Belgium), and two strains of Lactiplantibacillus plantarum (formerly Lactobacillus plantarum) previously isolated from Brazilian cheeses. The strains will be cultivated until 1010 colony forming units (CFU)/mL and their viable or non-viable (121ºC, 15 min) forms will be used in the adsorption assays in duplicate at 30o C under different pH conditions (3.0, 4.0, 5.0, 6.0, 7.0, and 8.0), contact times and FB1 concentrations (5.0, 10.0, 15.0 µg/mL). Samples will be shaken and centrifuged, and the supernatants analyzed by high/ultra-performance liquid chromatography coupled with a fluorescence and/or mass spectrometry detector (UHPLC-FLD/MS). The adsorption rate will be measured by correlating the peak area of the samples with the peak area of the positive and negative controls. The methods and techniques learned in the internship will be taught to colleagues in the home laboratory. In addition, the results will be published as scientific papers in peer-reviewed international journals and presented at national or international conferences. The results will elucidate the FB1 adsorption potential of the microorganisms, aiming at future applications in in vivo experiments.

News published in Agência FAPESP Newsletter about the scholarship:
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