ANTIMICROBIAL ACTION OF BLACKBERRY EXTRACT ON Streptococcus mutans

• Grant number: 24/02689-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2024
• End date: May 31, 2026
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Luciane Dias de Oliveira
• Grantee: Lais Zanferrari Sauer
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Caries is a multifactorial disease that depends on the following factors: I) Host; II) Time; III) Cariogenic Diet; IV) Etiological Agent. The focus of this work is the control of the etiological agent, Streptococcus mutans, through the possible antimicrobial phytotherapy activity of the blackberry extract, aiming to control caries disease. This work aims to: I) produce and chemically evaluate the Rubus subg rubus (blackberry) extract; II) Evaluate the antimicrobial and antibiofilm action on an ATCC strain and a clinical strain of Streptococcus mutans. For this purpose, the extract will be prepared from the leaves of a mulberry tree and the vehicle used will be absolute ethanol and ultrapure water. To determine antioxidant activity, the soluble solids content will be considered. Then the total flavonoids and phenols will be determined. Antimicrobial activity will be analyzed by broth microdilution tests to determine the minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC), and tests on monotypic biofilms with a contact time of 5 minutes by MMT analysis. The data will be subjected to normality tests and analyzed by Anova one-way and Tukey or Kruskal Walis and Dunn considering the level of significance (±e0,05).