Advanced search
Start date
Betweenand

Modulation of miR-146b expression by gene editing with CRISPR/Cas9 in papillary thyroid carcinoma

Grant number: 24/04546-2
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Start date: August 01, 2024
End date: July 31, 2025
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal Investigator:Edna Teruko Kimura
Grantee:Geovanna de Paula
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The CRISPR/Cas9 methodology has emerged as a promising tool for the precise modification of DNA sequences throughout the genome. Recently, the Casgevy, an CRISPR/Cas gene editing therapy in patients with sickle cell anemia and thalassemia, was approved in the United Kingdom. In the oncological context, CRISPR/Cas9 has been applied in the experimental study of cancers including thyroid cancer (TC) in modulating coding and non-coding genes in in vitro and animal models. Papillary thyroid carcinoma (PTC) is the most frequent histotype and presents alterations in both coding and non-coding genes, such as microRNAs (miRNAs). Among all dysregulated miRNAs in PTC, miR-146b is the most highly expressed, being associated with tumor aggressiveness and survival. In this project, we aim to evaluate the silencing of the oncomiR miR-146b through gene editing using different sgRNAs to modify the MIR146B gene, located on chromosome 10, employing the CRISPR/Cas9 system in a PTC cell line. We will analyse the level expression level of genes modulated by miR-146b in different clones generated after silencing. In addition to assessing the effect of silencing on miR-146b expression, we will investigate the influence on cell proliferation, migration, and viability. The application of CRISPR/Cas9 to modulate miR-146b opens up a new possibilities for understanding the role of this microRNA in the tumor biology of PTC and its clinical applications in the future.

News published in Agência FAPESP Newsletter about the scholarship:
More itemsLess items
Articles published in other media outlets ( ):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)