Effects of full-spectrum cannabis extract on gene expression of canine mesenchymal stem cells

• Grant number: 24/11155-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2024
• End date: August 31, 2025
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Rogério Martins Amorim
• Grantee: Yasmin Helena Lucci
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The diagnosis of immune-mediated diseases has become increasingly common in Veterinary Medicine, increasing the use of immunomodulatory substances to promote a better quality of life for the animal, given that they have no cure. The drugs most used to treat these diseases are glucocorticoids, which have multiple long-term adverse effects. Because of this, therapeutic alternatives, such as therapy with mesenchymal stem cells and phytocannabinoids, have been studied for their immunomodulatory/anti-inflammatory effects. This study aims to evaluate whether the stimulation of canine mesenchymal stem cells from adipose tissue with full-spectrum cannabis extract can increase their anti-inflammatory/immunomodulatory effect in vitro, when stimulated at high and low concentrations. The MTT method (3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide) will be used to determine the IC50 of the extract and the concentrations to be used in the study. Data relating to gene expression of inflammatory cytokines and neurotrophic factors, morphology and cell viability will be evaluated.