3D PRINTING OF FISH SKIN COLLAGEN SKIN DRESSINGS WITH THE ADDITION OF BIOACTIVE MOLECULES EXTRACTED FROM MICROALGAE

• Grant number: 24/06598-0
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): October 01, 2024
• Effective date (End): September 30, 2026
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Ana Claudia Muniz Renno
• Grantee: Lais Caroline Souza e Silva
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil

Abstract

Skin tissue plays a crucial role in protecting the human body, however, due to constant exposure to injuries of various origins, the healing of visible wounds becomes a complex and unnecessary process in terms of health treatments. The search for alternative treatments such as curatives has grown. Collagen (COL), especially marine collagen, has been an effective biomaterial for these dressings, promoting tissue healing and regeneration. The addition of secondary metabolites from microalgae intensifies the antimicrobial activity of the dressings. 3D printing of COL-based dressings has shown promising results. This study aims to manufacture skin COL skin dressings through 3D printing, incorporating fish and microalgae bioactive molecules to enhance their characteristics and biological activity. The objective is to evaluate the effectiveness of dressings in cell regeneration, antimicrobial activity and cellular prediction, as well as to analyze their physicochemical properties and durability. The study will use the species Syacium spp. to extract COL from your skin with a protocol adapted by the laboratory's scientific group. In parallel, the microalgae Chlorella vulgaris will be cultivated to obtain secondary metabolites with antimicrobial potential. The dressing will be manufactured by 3D printing, combining COL and sodium alginate. Characterization involves electron microscopy, infrared spectroscopy, hydration profile, porosity, pH and manipulation analyses. Tests include in vitro experiments, antimicrobials, antioxidants, metabolic and controlled cellular activity and genotoxicity.