Effect of a Chlorine-Releasing Product-Based Solution on the Color Change, Surface Roughness, and Microhardness of Dental Enamel - An In Vitro Study

• Grant number: 23/14376-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2024
• End date: October 31, 2025
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Fernanda de Carvalho Panzeri
• Grantee: Paula Yhorrana Viana Telles
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

This study aims to evaluate the antibiofilm potential of a chlorine-releasing solution and its in vitro effect on the physical and mechanical properties of dental enamel. The antibiofilm action will be tested on a mixed-species biofilm composed of three microbial species (Staphylococcus aureus, Candida albicans, and Streptococcus mutans). For biofilm formation, bovine tooth fragments (6 mm x 6 mm x 2 mm) will be planed and polished using abrasive papers in descending granulation order (600, 1200, and 2000). After biofilm maturation, the fragments will be immersed in a chlorine-releasing solution (Granudacyn, Mölnlycke Health Care), 0.12% chlorhexidine (CHX) (positive control), and phosphate-buffered saline (PBS) (negative control) for 5 minutes. The antibiofilm activity will be assessed by counting the number of colony-forming units per milliliter (CFU/mL) for each species individually. For the analysis of color, surface roughness, and microhardness, new bovine enamel fragments will be obtained. After initial readings, the fragments will be separated into five groups: Group 1: Immersion in saliva; Group 2: Immersion in chlorine-releasing solution; Group 3: Spray with chlorine-releasing solution; Group 4: Immersion in 0.12% CHX; Group 5: Spray with 0.12% CHX. The treatments will be performed for 30 seconds, and for spray application, six sprays will be applied to the surface of each fragment. After treatment, the samples will be washed and maintained in artificial saliva at 37 °C. New readings of color, roughness, and microhardness will be taken after 1, 7, 15, and 30 days of treatment. The changes in color (¿E00), surface roughness (¿Ra), and relative microhardness (¿KHN) will be calculated. The obtained data will be subjected to normality testing and appropriate statistical analyses