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Efficacy of toothpaste containing activated charcoal on the color change and effect on enamel surface roughness and wear

Grant number: 21/10164-7
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2022
Effective date (End): February 28, 2023
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Alessandra Buhler Borges
Grantee:Douglas Ferreira da Silva
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil


The whitening toothpastes are generally based on abrasives and/or chemical agents. The oral care industry has been developing a range of products used for extrinsic stains removal. Toothpastes containing activated charcoal are getting more popular, and its supposed mechanism of action is based on hindering pigment adsorption on the tooth surface. Despite their growing use, a lack of clarity regarding efficacy and safety is associated to such products, and studies with methodologies aimed at simulating clinical conditions with greater reliability are required. Thus, in this study, we will evaluate the effect of toothpastes containing activated charcoal on tooth color change and enamel surface roughness, considering their chemical (without abrasion) and chemical-mechanical (with abrasion) mode of action. In addition, surface enamel loss after prolonged brushing will be evaluated. Polished bovine enamel/dentin specimens will be divided into six treatments: deionized water (negative control - NC); Colgate® Maximum Anti-cavity Protection (Traditional toothpaste - positive control - PC); Colgate® Luminous White Activated Charcoal (LW); Oral-B® 3D White Therapy Charcoal (3DW); Curaprox® Black is White (BW); Dermavita® Whitemax (Activated Charcoal Powder - WP). In phase 1, the mechanisms of action involving or not abrasion (chemical and chemical-mechanical) will be tested. A staining/treatment cycle will be performed (immersion in staining solutions - 10 min, artificial saliva - 30 min and in toothpastes slurries - 2 min) 28 times, simulating four weeks of treatment. In chemical/mechanical challenge groups, abrasion will be performed in a brushing machine (15s - 200g) during the exposure to slurries. Color (L*a*b*) and surface roughness (Ra) will be measured by a reflectance spectrophotometer and a stylus profilometer, respectively. In phase 2, the abrasive potential of toothpastes will be evaluated by measuring the surface loss with profilometry. Additional specimens will be divided into the same groups tested in the phase 1 experiment. They will then be subjected to 100,000 abrasion cycles. Profilometric analyses will be performed at five periods: initial, after 25,000, 50,000, 75,000 and 100,000 cycles to determine the enamel surface loss (¼m). The data will be subjected to appropriate statistical analysis, according to the application of normality and homoscedasticity tests, at a significance level of 5%. (AU)

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Douglas Ferreira da. Effect of products containing activated charcoal on optical properties and dental surface. 2023. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos São José dos Campos.

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