Morphological characterization and protein expression of macrophage interaction to polydioxanone mesh - In vitro study

• Grant number: 24/07496-6
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: November 15, 2024
• End date: March 14, 2025
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Daniela Bazan Palioto Bulle
• Grantee: Ana Laura de Senne Zonta
• Supervisor: Benedetta Ghezzi
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Università degli Studi di Parma, Italy
• Associated to the scholarship: 23/05706-0 - FUNCTIONAL CHARACTERIZATION OF CLOT FORMED IN 3D PDO MATRIX - IN VIVO STUDY IN RATS, BP.IC

Abstract

Tissue regeneration is a complex process, involving a series of intricate phases that must be spatially and temporally coordinated. Each one of these phases, which involves different types of cells, growth and differentiation factors and signaling networks, plays a critical role in enabling and directing the subsequent phase. The use of absorbable and synthetic biomaterials, such as polydioxanone (PDO), has shown promising results when evaluating the regenerative process. A biomaterial that can be personalized in terms of physical and mechanical properties is ideal for achieving greater control of cell population during and after coagulation, consequently favoring regeneration. The macrophage, a blood clot component cell characterized by plasticity and versatility, has received considerable attention as an important and, in many cases, beneficial modulator of tissue remodeling. Macrophage polarization into a pro-inflammatory (M1) and/or anti-inflammatory (M2) phenotype is directly influenced by the stimuli received from the tissue microenvironment and the implanted biomaterial's surface. An orchestrated and efficient polarization is essential to achieve appropriate and functional remodeling. The aim of this study is to characterize the morphology and protein expression of macrophages cultured on 3D PDO mesh in vitro using Scanning Electron Microscopy (SEM) and Real-Time Quantitative Reverse Transcription PCR (qRT-PCR).