Petroselinum Crispum and contrast-induced nephrotoxicity in Wistar rats.

• Grant number: 24/16394-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2024
• End date: November 30, 2025
• Field of knowledge: Health Sciences - Nursing - Medical-Surgical Nursing
• Principal Investigator: Cassiane Dezoti da Fonseca
• Grantee: Raíssa Munhão Serra
• Host Institution: Escola Paulista de Enfermagem (EPE). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

The kidneys play a crucial role in maintaining internal homeostasis and eliminating undesirable substances, making them essential for overall health. Drug-induced nephrotoxicity, including contrast-induced nephropathy (CIN), can lead to acute and chronic kidney injuries, posing a significant clinical challenge. The pathogenesis of CIN involves direct toxic effects on renal tubules and hemodynamic disturbances. Phytotherapy, recognized by the Ministry of Health, presents a promising approach, with parsley demonstrating therapeutic potential. Studies indicate its antioxidant and anti-inflammatory efficacy, along with preventive properties against cancer and kidney stones. Research on phytotherapy in nephrotoxicity is vital for informing health policies and developing safe and effective treatments. Such findings can facilitate the integration of phytotherapy into healthcare systems, promoting natural and accessible therapeutic options for the population.Objective: To evaluate the effect ofPetroselinum crispum in animals subjected to acute kidney injury induced by contrastnephrotoxicity. Methodology: Twenty-four male adult Wistar rats weighing between 250-290 gwill be utilized, randomized into the following groups: a) Saline (Control) (n=5): animalsreceiving 1ml of 0.9% saline intraperitoneally (i.p.) for five days; b) Iodinated contrast (IC)(n=7): animals receiving 3ml/kg/day of ioversol 300mg/ml i.p. for five days; c) Saline +Petroselinum Crispum (PC) (n=5): saline animals receiving 7g/kg/day of PC aqueous solutionorally for five days; d) IC+PC (n=7): animals receiving iodinated contrast i.p. and PetroselinumCrispum orally for five days. Physiological parameters such as weight, water and food intake,kidney weight-to-body weight ratio, renal function (creatinine clearance), chemical analysis ofplasma levels of creatinine, urea, proteinuria, urinary peroxides, and IL-6 will be evaluated, andtheir results will be expressed as mean ± standard deviation. Variance between groups will beanalyzed using One Way ANOVA, followed by Tukey's multiple comparison post-test using thestatistical program Graph-Pad Prism version-8 for Windows®. Significance will be considered atp < 0.05. Expected results: Research on phytotherapy in nephrotoxicity is essential to elucidatepathophysiology and foster the development of safe and effective treatments. The integration ofphytotherapy with healthcare systems is highlighted, promoting innovative low-cost therapeutic options.