Protective effect of a solution containing a new recombinant protein from orange (CsinCPI-2) for the prevention of initial dental erosion in vitro

• Grant number: 24/15238-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2024
• End date: October 31, 2025
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Vinícius Taioqui Pelá
• Grantee: Wellington Alexandre de Melo Pursino
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

A cystatin derived from sugarcane has demonstrated efficacy in protecting against dental erosion and erosive tooth wear. Recently, within the same context, a new recombinant protein derived from orange, named CsinCPI-2, was produced. However, the potential use of CsinCPI-2 in Dentistry, specifically concerning dental erosion, has not yet been investigated. Therefore, this study aims to evaluate the protective effect of CsinCPI-2 and determine its optimal concentration for preventing initial dental erosion of enamel in an in vitro model. To achieve this, 105 bovine enamel specimens (4 x 4 mm) will be prepared and divided into 7 groups according to the following treatments (n/group=15): 1) Deionized water (negative control); 2) Commercial solution Elmex" (positive control); 3) 0.1 mg/mL of the sugarcane-derived protein (CaneCPI-5; organic positive control); 4) CsinCPI-2 at a concentration of 0.01 mg/mL; 5) CsinCPI-2 at a concentration of 0.1 mg/mL; 6) CsinCPI-2 at a concentration of 0.5 mg/mL; and 7) CsinCPI-2 at a concentration of 1.0 mg/mL. Initially, the specimens will be treated with the respective solutions (250 µL, 2 hours, 37°C, 250 rpm). Next, the Acquired Pellicle will be formed (250 µL, 2 hours, 37°C, 250 rpm) by the addition of unstimulated human saliva. Subsequently, the erosive challenge will be performed (1 mL, 0.65% citric acid, pH = 3.4, 1 min, 25°C, 250 rpm). These procedures will be conducted once a day for 3 days. Surface microhardness analyses and surface reflection intensity analysis will be performed (before and after the experiment), and surface changes will be calculated. The data will be analyzed for normality (Kolmogorov-Smirnov test) and homogeneity (Bartlett's test) to select the appropriate statistical test. The significance level will be set at 5%.