Biological behavior of titanium obtained by different printing parameters

• Grant number: 24/10609-7
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Start date: January 23, 2025
• End date: June 29, 2025
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Andréa Cândido dos Reis
• Grantee: Livia Maiumi Uehara
• Supervisor: Conrado Aparicio
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Universitat Politècnica de Catalunya (UPC), Spain
• Associated to the scholarship: 23/11966-5 - Surface characterization and microbiological behavior of titanium obtained by different printing parameters, BP.MS

Abstract

Understanding the osteogenic potential of titanium implants obtained by the additive manufacturing (AM) technique at different angles may guide the development of precision and individualized devices for specific applications. With the advances that have been made in titanium surface engineering and medical/dental AM techniques, the time has come to integrate the various areas of knowledge through transdisciplinarity. While most current studies are based on the development of different surface treatments, the boldness of this project lies in using technologies to determine which titanium microstructure parameters, generated by the laser sintering process, are capable of favoring bone biology. The aim of this study will be to evaluate the osteogenic potential of titanium surfaces obtained using the Powder Bed Fusion - Laser Beam (PBF-LB) AM technique at different angles. The titanium alloy (Ti6Al4V) samples will be obtained using two methods: Conventional machining and AM - PBF-LB. The printed disks are obtained at two different angles (0° and 45°). After sterilization, human mesenchymal stromal cells will be cultured and osteogenic potential will be assessed by the following methods: Cell viability analysis by DAPI staining and cell counting by fluorescent microscopy; Identification and quantification of osteocalcin and/or osteopontin by ELISAs; Evaluation of the formation and quantification of mineralized extracellular matrix by Alizarin Red S. The results obtained will be subjected to parametric or non-parametric tests, depending on the distribution of the data (±=0.05).