Scaffolds based on collagen, carrageenan and doxycycline in a subantimicrobial dose for in vivo treatment of periodontal disease

• Grant number: 24/09087-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: January 01, 2025
• End date: December 31, 2027
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Ana Paula Ramos
• Grantee: Tatiane Cristina Dotta
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 19/08568-2 - Investigation of the extracellular vesicles (VEs) role in the initiation, propagation, regeneration, and modeling of biological mineralization, AP.TEM

Abstract

The therapeutic approach combined with regenerative and antimicrobial materials along with scaling and root planing (SRP) presents itself as a promising perspective for the treatment of periodontal disease. This project proposes a randomized, controlled, and double-blind clinical study aimed at developing and evaluating the regenerative effects of materials based on type II collagen (C-II), carrageenan (Car), and subantimicrobial dose doxycycline (SDD), applied intrapocket, compared to metronidazole (MDZ), the most commonly used medication for this purpose. The scaffolds will be prepared and characterized using Scanning Electron Microscopy, Fourier Transform Infrared Spectroscopy, antimicrobial release, their effects on human gingival fibroblast and osteoblast cultures in vitro, osteogenic capacity, and antimicrobial efficacy against periodontal pathogens. Forty patients with periodontitis will be randomly allocated into four groups (n = 10): G1 - SRP alone (control); G2 - SRP + C-II and Car scaffolds (negative control); G3 - SRP + C-II, Car, and SDD scaffolds; and G4 - SRP + C-II, Car, and MDZ scaffolds (positive control). The concentrations of SDD and MDZ in gingival crevicular fluid will be monitored using high-performance liquid chromatography. Additionally, clinical indicators of periodontitis (probing depth, clinical attachment level, and bleeding on probing), radiographic parameters, and microbial diversity will be assessed. Evaluation periods will include: T0, T2 (48 hours after SRP and scaffold application), T4 (96 hours), T7 (7 days), T15 (15 days), T30 (30 days), T60 (60 days), T90 (90 days), and T180 (180 days post-treatment). Statistical analysis will be conducted using R software (p<0.05). The aim is to obtain biomaterials that contribute to periodontal treatment and bone regeneration, reducing bacterial resistance and associated costs.