Development and characterization of mango popsicles enriched with beta-carotene using the microalgae Dunaliella salina

• Grant number: 24/17597-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2025
• End date: December 31, 2025
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Technology
• Principal Investigator: João Carlos Monteiro de Carvalho
• Grantee: Nicole Loredo Faustino
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 22/07002-8 - Genetic modification and evaluation of plant hormones in beta-carotene production by Dunaliella salina in continuous cultivation process, AP.R

Abstract

Microalgae can be considered an alternative, sustainable, and promising food component due to their diversity in bioactive compounds and low environmental impact cultivation. In this context, the microalga *Dunaliella salina* stands out as a rich natural source of beta-carotene, an essential compound for human health due to its antioxidant, immunomodulatory properties, and its role as a precursor to vitamin A. In this sense, this study proposes the development and characterization of mango popsicles enriched with the biomass of *Dunaliella salina*, aiming to increase the intake of beta-carotene and other bioactive carotenoids. Additionally, the combination of mango, a nutrient-rich fruit, with milk is explored, as it optimizes the absorption of fat-soluble vitamins. The methodology used includes the production of *D. salina* biomass, formulation of popsicles with different concentrations of microalga, and detailed analysis of biochemical composition, carotenoid bioaccessibility, antioxidant properties, and sensory acceptability. The innovation also addresses sustainability aspects, such as CO2 fixation by the microalga, contributing to the reduction of greenhouse gas emissions. Therefore, the project aims to offer a sustainable and nutraceutical solution for beta-carotene supplementation in the diet, with the purpose of improving human health and meeting the demand for functional foods.