Double Labeled Water Method for determining energy expenditure in growing dogs.

• Grant number: 24/22701-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2025
• End date: March 31, 2026
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Aulus Cavalieri Carciofi
• Grantee: Yasmin Gonçalez Sasaki
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Dogs spend a daily amount of energy to maintain their organic functions, being extremely important for its development and maintenance. The lack or excess energy can result in worrying situations, leading to possible malnutrition or obesity, respectively, presenting critical during growth when inadequate. Therefore, knowledge about thedaily energy expenditure (GE) is necessary, especially in animals that are in its initial phases of development, in which inadequate energy is resulting in a dysfunctional and unhealthy adult. There are different ways to evaluate GE, through indirect calorimetry, food consumption per day and through stable isotopes. The present study aims to compare the application of different GE estimation methods, the consumption method (CA)of food and stable isotope method of ADM in growing dogs. 12 Beagle dogs will be used, 6 males and 6 females during the first 12 months of age, with AC assessment over 12 months, applying the consumption in grams per day as a function of time in weeks in a curve model Gompertz growth. At ages 12 weeks and 6, 9 and 12 months of age, applied the DLW protocol to estimate GE via blood collection in basal times, 3 and 5 hours and 3, 4 and 5 days after application of the isotope solution for determination of the enrichment and decay of deuterium and oxygen 18, analyzed in desorbed samples by ratio mass spectrometryisotopic. The study will be conducted in a completely randomized design with time as a repeated measure, organized in a 2 factorial arrangement (GE methods) x 4 (ages), totaling 8 treatments, comparing GE methods, ages and its interaction method and age. If there are differences in the F test, averages will becompared using the Tukey test and limits of agreement established in accordance with Bland and Altman (1986) applied to the comparison of methods, added to Pearson correlation. Values ¿¿of P less than 0.05 will be considered significant.