INFLUENCE OF DIFFERENT EXERCISE MODALITIES ON PERIPHERAL MUSCLE ASPECTS OF METABOLIC SYNDROME

• Grant number: 24/19053-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2025
• End date: June 30, 2026
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Francis Lopes Pacagnelli
• Grantee: Natália Cervantes Uzeloto Guazi
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil

Abstract

Introduction: Cardiovascular risk factors constitute the Metabolic Syndrome (MS) and promote adaptive responses in skeletal muscle that include changes in the Extracellular Matrix (ECM). Changes in the ECM are related to reduced transmission of contraction force, flexibility and cause fatigue that worsens quality of life and increases the risk of fractures and death. As a non-pharmacological treatment for MS, aerobic (ABE) and resistance (RE) exercises stand out. However, there are no studies comparing these two types of exercises in the molecular aspects of the muscle ECM in rats with MS. Objective: To investigate ME in catabolic conditions such as MS and the influence of two types of physical exercise. Methods: Wistar rats (n = 40) were randomly distributed into two groups (n = 20), Control Group (C) receiving control chow + water (control diet [CD]) or High Sugar Fat Group (HSF) receiving chow rich in simple carbohydrates and fat + drinking water with 25% sucrose added. The animals received the diets throughout the experimental period, and in the 20th week they were subjected to analysis of the components of MS. The animals were redistributed into four experimental groups (n = 10), Control, MS, MS + EFA, and MS + EFR. The experimental period lasted 30 weeks. The gene expression of collagen and metalloproteinase in the soleus and extensor digitorum longus (EDL) muscles will be evaluated. The normality of the data will be assessed by the Kolmogorov Smirnov test. For comparison between groups of parametric data, ANOVA followed by Tukey's post-test or Kruskal-Wallis followed by Dunn's post-test will be used. P <0.05 will be considered significant. The aim of this research is to promote a better understanding of the ECM of skeletal muscle in MS and after aerobic and resistance training to expand knowledge in the field of muscle physiology. (AU)