Evaluation of Hygiene Protocols Applied to Complete Dentures Obtained by Additive Manufacturing Compared to Conventional Manufacturing - Crossover, Randomized and Controlled Clinical Study

• Grant number: 25/10451-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2025
• End date: March 31, 2028
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Cláudia Helena Lovato da Silva
• Grantee: Beatriz de Camargo Poker
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 24/13882-6 - Complete dentures obtained by additive manufacturing compared to conventional manufacturing - Crossover, randomized and controlled study, AP.R

Abstract

This randomized, controlled, crossover clinical study aims to compare complete dentures obtained by additive manufacturing with complete dentures obtained by conventional methods through variables centered on the biological and physical behavior of the materials. Thus, the antibiofilm efficacy of hygiene protocols will be compared according to the type of denture manufacturing material. Totally edentulous individuals (n=26) who meet the eligibility criteria will use complete dentures manufactured by additive manufacturing (PT3D) and manufactured by the conventional method (PTC), in a randomized and crossover manner. During the experimental period, participants will be instructed to use hygiene protocols for 14 days [brushing and immersion in 0.25% sodium hypochlorite (EHS) and brushing and immersion in 0.15% triclosan (ET)]. The protocols will be instituted in a randomized and crossover manner with a 7-day washout period. Response variables will be measured at Baseline (microbial load of the prosthesis in use and measurement of the color of the prosthesis to be installed), after 2 months of prosthesis installation (color and microbial load by culture and counting of colony-forming units and by the FISH method - fluorescence in situ hybridization) and after the use of hygiene protocols for analysis of antibiofilm efficacy (color and removal of biofilm by area, identification and microbial load by culture and colony-forming units, cellular metabolism, formation of pseudohyphae/hyphae of Candida spp.). Data will be assessed for normality (Shapiro-Wilk test) and homoscedasticity (Levene test) to define the statistical tests to be used, considering a 95% confidence interval (SPSS 21.0 software).