Evaluation of the anti-inflammatory and tyrosinase-inhibiting activity of a blend of Mediterranean extracts for application in formulations for the treatment of post-inflammatory hyperpigmentation.

• Grant number: 25/25467-6
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: February 02, 2026
• End date: May 01, 2026
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Patrícia Maria Berardo Gonçalves Maia Campos
• Grantee: Talita de Moraes Pinheiro
• Supervisor: Luis Antonio Monteiro Rodrigues
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Universidade Lusófona, Campus Lisboa, Portugal
• Associated to the scholarship: 25/12501-1 - Development of dermocosmetic formulations containing a blend of Mediterranean extracts for the treatment of post-inflammatory hyperpigmentation, BP.IC

Abstract

Post-inflammatory hyperpigmentation (PIH) is a skin disorder that occurs as a response to inflammatory processes such as acne. Consequently, the search for effective treatments, especially those containing naturally derived ingredients, has become an increasing demand from both the cosmetic industry and consumers. In this context, a Mediterranean Extract Blend (MEB) has emerged as a promising alternative. To validate the results obtained from clinical efficacy studies conducted in Brazil, it is essential to understand the mechanisms of action that justify the observed effects. Therefore, the present project aims to investigate the skin retention of the bioactive compounds present in the MEB, as well as to evaluate its anti-inflammatory, antioxidant, and tyrosinase inhibitory activities using an ex vivo human skin model. The methodology will include skin penetration studies using Franz diffusion cells, enzymatic assays to assess the inhibition of tyrosinase, collagenase, and elastase, as well as cytotoxicity and antioxidant activity tests in keratinocyte and melanocyte cultures. The data generated are expected to scientifically support the clinical results obtained in Brazil, strengthening the development of innovative and effective dermocosmetics backed by a well-established mechanism of action. (AU)