Biocurative for Chronic Wounds using Nanotechnology from the microorganisms Ganoderma lucidum, Grifola frondosa and Trametes versicolor.

• Grant number: 25/11982-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2025
• End date: November 30, 2026
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Paulo Noronha Lisboa Filho
• Grantee: Nicolli Fernandes Muniz
• Host Institution: Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil

Abstract

Chronic wounds represent a significant challenge for healthcare systems worldwide, as their characteristics, such as high prevalence and the reduction in patients' quality of life, result in severe discomfort and high healthcare costs. The ideal type of dressing should have good breathability and biocompatibility, maintain moisture at the wound site while removing excess exudate, and exhibit low cytotoxicity and high healing capacity. Despite current advances in the development of wound dressings, polymeric and naturally derived compounds are considered attractive alternatives for wound care. In this context, chitosan (CS) is a natural polymer that shows superiority over many biopolymers, and polyvinyl alcohol (PVA) is another polymer employed as a main component in wound dressings. Alongside the promising results already obtained with PVA/CS dressings, the incorporation of microorganisms is a convenient and cost-effective solution for preventing and treating wound infections. In this context, the fungi Ganoderma lucidum, Grifola frondosa, and Trametes versicolor are advantageous for wound treatment due to their numerous beneficial properties. The objective of this research is the electrospinning; a simple, versatile, and low-cost nanotechnology technique-of the culture supernatants from these microorganisms, incorporated into PVA and CS, and the corresponding evaluation of the healing potential of this bio-based dressing, along with the determination of the electrospinning experimental parameters for obtaining chitosan, PVA, and composite fibers, characterizing these fibers both chemically and morphologically.