Methylation pattern in the candidate molecular markers in giant cell tumor of bone

• Grant number: 09/08829-9
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): August 01, 2009
• Effective date (End): July 31, 2010
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Paula Rahal
• Grantee: Andre Luis Giacometti Conceicao
• Home Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

The giant cell tumor of bone (GCT) is a destructive osteolytic neoplasm that represents 5% of all the primary bone tumor, although benign presents a aggressive local behavior. The tumor shows specific histological features, being composed by mononuclear cells and multinuclear giant cells with variable potential for growth. Even the GCT with benign characteristics can evolve with local metastases, pulmonary metastases, in lymph nodes and more rarely malignant transformation. Studies involving analysis of gene expression has identified several genes whose expression is reduced in some types of tumors, as carcinoma of head and neck and clear cell renal carcinoma. Many molecular mechanisms may be involved in the differential expression of these genes in tumor tissue. This project has the aim of evaluating the methylation profile of the genes DUSP1, CSTB, GPC3, KTN1 and ZAK showing low expression in GCT and some other tumor types already described in literature, through MSP technique, which offers a qualitative assessment of the methylation state of CpGs islands. Thus, the assessment of the methylation profiles of selected genes may contribute to the development of more effective diagnosis and prognosis for giant cell tumor, and for developing new anti-neoplastic drugs. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GIACOMETTI CONCEICAO, ANDRE LUIS; BABETO, ERICA; CANDIDO, NATALIA MARIA; FRANCO, FERNANDA CRAVEIRO; PIRES DE CAMPOS ZUCCARI, DEBORA APARECIDA; BONILHA, JANE LOPES; CORDEIRO, JOSE ANTONIO; CALMON, MARILIA FREITAS; RAHAL, PAULA. Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone. JOURNAL OF CANCER, v. 6, n. 7, p. 593-603, 2015. Web of Science Citations: 1.