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Mutation patterns in gag cleavage sites and their associations with protease activity and antiviral therapy in HIV-1 infection

Grant number: 07/04890-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2007
Effective date (End): September 30, 2009
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Ester Cerdeira Sabino
Grantee:Evelyn Regina de Souza Pastena
Home Institution: Hemocentro de São Paulo. Fundação Pró-Sangue. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

The ultimate objective of this study is to determine the incidence and associations of mutations within gag and protease in order to search for criteria which permit interpretation of genetic variations capable to interfere in the catalytic activity of the protease enzyme, modify viral infectivity dynamics and contribute to the emergence of viral resistance to protease inhibitors in a well characterized cohort of HIV recently infected individuals in the state of São Paulo. A total of 150 samples of whole blood will be collected from HIV recently infected individuals as defined by other studies on the base of STARH algorithmic criteria. The region flank the whole gag gene will be amplified after synthesis of cDNA by using RT-PCR protocol established in our laboratory. Reaction products will be sequenced and and each sequence will be processed and analysed. The informations of sequence alignments, epidemiological and socioepidemiological data, viral load, CD4 T cell count and antiretroviral resistance will be compared and incorporated to the results.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SANABANI, SABRI SAEED; DE SOUZA PASTENA, EVELYN REGINA; KLEINE NETO, WALTER; MARTINEZ, VANESSA POUZA; SABINO, ESTER CERDEIRA. Characterization and frequency of a newly identified HIV-1 BF1 intersubtype circulating recombinant form in Sao Paulo, Brazil. VIROLOGY JOURNAL, v. 7, APR 16 2010. Web of Science Citations: 18.
SANABANI‚ S.S.; PASTENA‚ ER; NETO‚ W.K.; MARTINEZ‚ V.P.; SABINO‚ E.C.; OTHERS. Characterization and frequency of a newly identified HIV-1 BF1 intersubtype circulating recombinant form in Sao Paulo‚ Brazil. VIROLOGY JOURNAL, v. 7, p. 74, 2010.

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