Estudo comparativo do aculo hepatico de cobre em bovinos e bubalinos

• Grant number: 06/06443-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2007
• End date: May 31, 2008
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Enrico Lippi Ortolani
• Grantee: Rodrigo Nogueira Fernandes Ferreira
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The main aim of this project is to carry out a comparative study of the susceptibility of cattle (crossbred) and buffalo (Murrah) to accumulative copper poisoning, by drenching increasing doses of copper, studying liver Cu concentration. Ten cattle and 10 buffalo steers will be randomly distributed in a control (n=4) and copper supplemented group (n=6). The last group will be initially drenched with 2 mg Cu/kg/BW (as CuSO4.5H2O) daily for a week; every following week, up to the end of the experiment (90th d), this initial dose will be increased of 2 mg Cu/kg/BW. For three times during the experiment (before the 1st drenching, 45th and 90th d) a liver biopsy will be taken to evaluate the degree of copper accumulation in this stocking organ. A fragment of liver will be keep in formol (10%) to perform histopathological analysis, using Hematoxilin-eosin, Rodamin and Pearls, with the object of evaluated morphological changes and copper accumulation in the hepatocytes