Evaluation of Photodynamic Therapy with LED in the process of cell adhesion

• Grant number: 10/11889-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: September 01, 2010
• End date: August 31, 2012
• Field of knowledge: Biological Sciences - Biophysics - Radiology and Photobiology
• Principal Investigator: Cristina Pacheco Soares
• Grantee: Geisa Rodrigues Salles
• Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil

Abstract

Photodynamic therapy (PDT) is a therapeutic modality for the treatment of neoplastic diseases and non-neoplastic characterized by overgrowth of unwanted or abnormal cells. This therapy involves the activation by light in the presence of molecular oxygen, certain dyes (photosensitizers) that are somehow selectively absorbed by the target tissue. This process has been used as a palliative technique in treating cancer in its advanced stage, when other options do not show satisfactory results, but its application has proved to be appropriate for small lesions and / or superficial lesions due to low penetration of light .. The change in adhesion of cancer cells to a substrate or cells is another important consequence of PDT. Numerous adhesion molecules are involved in this process and the abnormal expression of these molecules can lead to an increase or decrease of potential metastásico.No this work will be an investigation of the process of cell adhesion and its influence on the change of potential metastatic cell lines HEp-2 ( human laryngeal carcinoma) and M3 (human breast adenocarcinoma) who underwent PDT with different photosensitizers and light source LED. A variety of tests will be performed in order to help understand changes in cell-cell adhesion, cell matrix and resistance to treatment with trypsin.