Evaluation of the tissue response to silver nanoparticles

• Grant number: 09/16069-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2009
• End date: November 30, 2010
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: João Eduardo Gomes Filho
• Grantee: Fernando Oliveira Silva
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The aim of this study was to evaluate the tissue response to implanted polyethylene tubes filled with fibrin sponge embedded with silver nanoparticles dispersion. Thirty rats will individually receive four polyethylene tubes, two filled with sponge embedded in 47ppm or 23ppm silver nanoparticles dispersion, one filled with fibrin sponge embedded with 2,5% sodium hypochlorite and one more empty tube as control. The observation periods will be 7, 15, 30, 60 e 90 days. After each period of time, six animals will be killed and the tubes and surrounding tissue removed, fixed and prepared to be analyzed in light microscope with glycol methacrylate embedding, 3 mm serial cutting and HE staining. Scores will be defined as follows: 0, none or few inflammatory cells, no reaction; 1, <25 cells, mild reaction; 2, 25 to 125 cells, moderate reaction; 3, >125 cells, severe reaction. Fibrous capsule was categorized as "thin" when thickness was < 150 ¼m and "thick" at > 150 ¼m. Necrosis and formation of calcification were recorded. The data will be statistically analyzed using Kruskal Wallis test.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES-FILHO, JOAO EDUARDO; SILVA, FERNANDO OLIVEIRA; WATANABE, SIMONE; ANGELO CINTRA, LUCIANO TAVARES; TENDORO, KARINA VANESSA; DALTO, LUANA GODOY; PACANARO, SARA VIEIRA; LODI, CAROLINA SIMONETTI; FERREIRA DE MELO, FERNANDA FRAGOSO. Tissue Reaction to Silver Nanoparticles Dispersion as an Alternative Irrigating Solution. JOURNAL OF ENDODONTICS, v. 36, n. 10, p. 1698-1702, OCT 2010. (09/16069-4)