Mass spectrometry lipidomic analysis in bovine oocytes and embryos

• Grant number: 08/10756-7
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): May 01, 2009
• Effective date (End): August 31, 2010
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marcos Nogueira Eberlin
• Grantee: Christina Ramires Ferreira
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

In vitro produced bovine embryos have lower quality, are less viable and more cryosensitive than in vivo derived embryos. Nowadays, expansion of commercial IVF main hindering is to obtain satisfactory pregnancy rates from cryopreserved IVF Zebu embryos. Many researches reported the relationship of lipids with fertility, their role in embryo development and cryosensitivy. The main objective of this project is to stablish a profile of the most abundant lipid classes, which are triglycerols (TAGs) and phospholipids, in bovine immature oocytes, in vitro matured oocytes, in vivo derived embryos, and in vitro produced embryos cultured with or without linoleic acid supplementation in culture medium. Harvested oocytes and embryos will have the zona pellucida eliminated by pronase 0.5% exposure, and will be stored in methanol/water 1:1 (v/v) solution at -80oC until analysis. Samples will be submitted to matrix-assisted laser desorption/ionization - time of flight mass spectrometry analysis (MALDI-TOF MS), using 2,5-dihydroxybenzoic acid (DHB) as matrix. Mass spectra collection will be performed in a MALDI-TOF (Micromass, Manchester, UK) mass spectrometer. Spectra will be processed (MassLynx v.4.0 Waters, Manchester, UK) in order to characterize the lipids and to perform chemiometric analysis (Pirouette 3.11, Infometrics Inc, EUA). Lipidomics of oocytes and embryos, which has not been yet reported in the literature, may contribute with strategies for cryopreserving in vitro produced bovine embryos. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, CHRISTINA R.; SARAIVA, SERGIO A.; CATHARINO, RODRIGO R.; GARCIA, JERUSA S.; GOZZO, FABIO C.; SANVIDO, GUSTAVO B.; SANTOS, LUIZ FERNANDO A.; LO TURCO, EDSON G.; PONTES, JOSE HENRIQUE F.; BASSO, ANDREA C.; et al. Single embryo and oocyte lipid fingerprinting by mass spectrometry. Journal of Lipid Research, v. 51, n. 5, p. 1218-1227, MAY 2010. (08/10756-7)

LO TURCO, EDSON GUIMARAES; MARTINS FERREIRA SOUZA, GUSTAVO HENRIQUE; GARCIA, JERUSA SIMONE; FERREIRA, CHRISTINA RAMIRES; EBERLIN, MARCOS NOGUEIRA; BERTOLLA, RICARDO PIMENTA. Effect of endometriosis on the protein expression pattern of follicular fluid from patients submitted to controlled ovarian hyperstimulation for in vitro fertilization. Human Reproduction, v. 25, n. 7, p. 1755-1766, JUL 2010. (08/10756-7)