Effect of fluoride on the osteoblastic differenciation-derived from mesenchymal bone marrow cells

• Grant number: 09/16402-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2010
• End date: December 31, 2010
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Marília Afonso Rabelo Buzalaf
• Grantee: Fabiana Amadeu de Oliveira
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Besides their anticariogenic role, fluoride (F) is involved in the bone metabolism. Several works has been shown their role in the bone formation, presenting a therapeutic role in osteodegeneratives diseases such as osteoporosis. In clinical trials, the therapy with F in osteoporotic patients was benefic in only 1/3 of them, suggesting genetic influences on F effect. However, less is known about the role of F in the proliferation and diferentiation of osteoblasts derived from bone marrow mesenchymal cells (MSC). MsC cells are able to differentiate in many cells depending on the environment, including osteoblasts, cells involved in the bone formation. Thus, the objective of this study is to evaluate the F effect in proliferation and differentiation of mesenchymal cells to osteoblasts obtained from different animals strains. For that, female A/J (susceptible to F effect in the bone mechanical proprieties) and 129P3/J (resistant to F effect in the bone mechanical proprieties). Msc will be collected and cultivated in vitro in osteogenic medium in a presence or not of F (NaF form) in different concentrations and evaluate after 1, 3, 7, 10 and 21 days. Proliferation and cell viability will be evaluate by cell count and MTT assay. The differentiation will be analysed by alkaline fosfatase measurement, and Runx-2, osteocalcin, osteopoetin, collagen and RANKL expression.